关键词: 硫丹, 维生素E, 小鼠, IL-2, IL-6, IFN-y

Abstract: To investigate the effects of vitamin E on the transcription of spleen IL-2，IL-6 and IFN一-y genes in mice treated with endosulfan．The treatment was assigned 3 groups．and the level of both endosuIfan and vitamin E were 0 mr,／(kg·d)and 0 mg／(kg·d)(control group)，0．8 ms／(kg·d)and 0 mg／(kg·d)(endosulfan-treatment group)，0．8 mg／(kg·d)and 100 mg／(kg·d)(experimental group)，respectively．Spleens were collected to be analyzed the mRNA of IL-2，IL-6．and IFN-．y genes by Real-Time PCR method．The results showed that the mRNA levels of IL-2 and IL-6 gene in the endosulfan-treatment group were significantly higher than that of control group； although IFN-^r mRNA level in endosulfan-treatment group had an increasing tendency compared with control group，there was no significant difference between those two groups．The mRNA levels of IL-2 and IL-6，in the experimental group were significantly lower than that of endosulfan—treatment group，while the mRNA level of IFN-1 gene had a decreasing tendency in experimental group compared with endosulfan-treatment group，but there was no significant difference between those two groups．The results indicated that endosulfan can induce the gene transcriptions of spleen IL-2 and IL-6，but there is no significant effect on the gene transcription of spleen IFN一1 in mice，while vitamin E can alleviate or partly alleviate the endosulfan actions．

Key words: Endosulfan, Vitamin E, Mice, IL-2, IL-6, IFN-y