关键词: Tyr03、Axl受体酪氨酸激酶, loxp位点, X．gal染色

Abstract: Abstract：Objective Use escherichia coli B·galactasc gene LacZ as report gene，established Tyr03，Axl receptor tyrosine kinases transgenic mouse containing loxp sites，mating with different tissue specific Cre mice to obtain space．time expressions of transgenic mice，make a foundation for research Tyr03 and Axl in each organization functions．Methods Constructing Tyr03，Axl receptor tyrosine kinase plasmid containing loxp sites，sequencing correctly，through microscopic injection technology to insert the Geo-STOP—msAxl and Geo—STOP。msTyr03 gene containing CAG promoter upstream into BDFl fertilized eggs，Transplant to ICR receptor mouse，Stay after breeding， using double primers PCR to appraisal Fo and Fn generation mouse genotype，using RT·PCR，X—gal staining to observe LacZ genes’express in mice organization，using WB to detect the protein expression in transgenic mice and wild type．Result Adopt double primer PCR to appraisal new born mice，get modified positive transgenic mouse， use the RT．PCR conformed the exist of LacZ gene；the x—gal staining，observed blue precipitation in the brain， testicular and the thymus of Geo—STOP—msTyr03。observed blue precipitation in brain，heart，ovarian，kidney of Geo· STOP．msAxl，indirectly illustrate the expression part of aim gene；For identification of double primers positive Geo· STOP．msTyr03。Geo—STOP．msAxl transgenic mice and wild type mice，we use the WB to validate the Axl and Tyr03 gene expressing no difference，and explain the exist of STOP sequence，so the Axl and Tyr03’8 expression is restrained．Conclusions Successful got Geo—STOP．msAxl and Geo·STOP—msTyr03 tyrosine receptor transgenic moUSe．

Key words: Tyr03, Axl receptor tyrosine, Loxp site, X—gal staining