淋巴细胞脉络丛脑膜炎病毒在实验大鼠中的感染状况

实验动物科学

淋巴细胞脉络丛脑膜炎病毒在实验大鼠中的感染状况

佟巍;乔红伟;魏强;张丽芳;代小伟;刘先菊;王艳荣;刘云波;

中国医学科学院北京协和医学院医学实验动物研究所;

收稿日期:2010-08-28 修回日期:2010-08-28 出版日期:2010-08-28 发布日期:2010-08-28

Infectious Investigation of Lymphocytic Choriomeningitis Virus(LCMV) in Laboratory Animal Rat

TONG Wei,QIAO Hong-wei,WEI Qiang,ZHANG Li-fang,DAI Xiao-wei,LIU Xian-ju,WANG Yan-rong,LIU Yun-bo

(Institute of laboratory Animal Science,Chinese Academy of Medical sciences and Peking Union Medical College,Beijing 100021,China

Received:2010-08-28 Revised:2010-08-28 Online:2010-08-28 Published:2010-08-28

摘要/Abstract

摘要： 目的对实验室留存的大鼠血清样本进行淋巴细胞脉络丛脑膜炎病毒(LCMV)抗体检测,调查LCMV在我国实验大鼠中的感染情况。方法依据现行国家标准中规定的免疫荧光试验(IFA)方法对我实验室保存的172份大鼠血清样本(包括48份无级别、100份清洁级和24份SPF级大鼠血清样本)进行LCMV抗体检测。结果无级别大鼠血清样本(n=48)15份阳性,阳性率为33.25%,强阳性样本滴度可达1∶1280;SPF级及清洁级大鼠血清样本全部阴性。结论大鼠可以感染LCMV,实验动物等级标准应增加对大鼠LCMV的检测。

关键词: 淋巴细胞脉络丛脑膜炎病毒(LCMV), 实验大鼠:免疫荧光试验

Abstract: Objective To investigate the infection status of LCMV in laboratory animal rat,we inspected LCMV antibody of rat serum samples preserved in our laboratory.Methods Immunofluorescent(IFA) from the current national standard was used in testing LCMV antibody of 172 rat serum samples from 48 non-quality controled animal,100 clean animal and 24 specific pathogen free animal respectively.Results 15 samples in non-quality controlled rat were tested as LCMV antibody positive(positive rate 33.25%),and the highest antibody 滴度 is 1∶ 1280;The samples of SPF and clean animal were tested as LCMV antibody negative.Conclusions The rat may be infected by LCMV,so we suggested that the LCMV should be added into microbiological quality control standard of rat as a monitoring item.

Key words: LCMV, Rat, IFA

佟巍;乔红伟;魏强;张丽芳;代小伟;刘先菊;王艳荣;刘云波;. 淋巴细胞脉络丛脑膜炎病毒在实验大鼠中的感染状况[J]. 实验动物科学.

TONG Wei,QIAO Hong-wei,WEI Qiang,ZHANG Li-fang,DAI Xiao-wei,LIU Xian-ju,WANG Yan-rong,LIU Yun-bo. Infectious Investigation of Lymphocytic Choriomeningitis Virus(LCMV) in Laboratory Animal Rat[J]. laboratory animal science.