关键词: 内毒素, 吸附柱, 灌流, 比格犬

Abstract: Objective To explore the efficacy and safety of hemoperfusion adsorption in dogs.Methods Beagle dog model of endotoxemia was established by intravenous infusion of endotoxin LPS,and was randomly divided into treatment group and control group respectively.Treatment group was given the perfusion adsorption therapy using hemoperfusion machine and endotoxin adsorption column,and the control group was treated with the non-endotoxin adsorbent column.Blood physiological and biochemical parameters and endotoxin content were measured at multiple time points.Results Safety tests: body temperature dropped continuously in the treated dogs,but rose at first and then fell in the control dogs.In 90 min and 120 min after perfusion,body temperature was significantly different between the control and treatment group(P<0.05).Compared with the control group,there were no significant differences in heart rate,blood routine,serum total protein and albumin,electrolyte concentration, prothrombin time,activated partial prothrombin time,serum complement C3 concentration,thrombin content,and so forth(P>0.05).Detection of adsorption efficiency: the level of serum endotoxin was decreased significantly in the treatment group(P<0.01).Conclusion Serum endotoxin can be adsorbed and the level of serum endotoxin can be reduced by the endotoxin adsorption column,which is of good blood compatibility.

Key words: Endotoxin, Adsorption column, Hemoperfusion, Beagle dog