关键词: 斑马鱼, 质量控制, 毒性实验

Abstract: Objective To study the toxicity of zebrafish in different health conditions exposed to the same chemical pollutant,and to discuss the suitability of current quality control(QC) of laboratory fish. Methods According to the OECD 203 Fish Acute Toxicity Test method,healthy and sick zebrafish were placed in methomyl and potassium dichromate(K_2Cr_2O_7) respectively.Results and Conclusion The values of K_2Cr_2O_7 24 h-LC_(50) and Methomyl 96 h-LC_(50) of the healthy fish were 323.38 mg/L and 4.91 mg/L respectively,and were 302.55 mg/L and 3.04 mg/L in the sick fish.Although the K_2Cr_2O_7 24 h-LC_(50) values of both fish meet the QC requirement,there is a significant difference on the 96h-LC_(50) values between the two groups of fish in the acute toxicity test of methomyl.

Key words: Zebrafish(Brachydanio rerio), Quality control, Toxicity test