三硝基丙酸诱导大鼠亨廷顿氏病模型的改进研究

实验动物科学

三硝基丙酸诱导大鼠亨廷顿氏病模型的改进研究

李峰;徐群渊;

首都医科大学北京神经科学研究所北京市神经再生修复研究重点实验室教育部神经变性病学重点实验室首都医科大学神经生物系;

收稿日期:2009-08-28 修回日期:2009-08-28 出版日期:2009-08-28 发布日期:2009-08-28

Study of the Rat Model of Huntington's Disease Induced by 3-Nitropropionic Acid

LI Feng,XU Qun-yuan

(Beijing Institute for Neuroscience,Capital Medical University;Beijing Center of Neural Regeneration & Repairing;Key Laboratory of Neurodegenerative Diseases of Ministry of Education of China,Beijing 100069,China

Received:2009-08-28 Revised:2009-08-28 Online:2009-08-28 Published:2009-08-28

摘要/Abstract

摘要： 目的评价两种不同的三硝基丙酸(3-Nitropropionic Acid,3-NP)给药方式所致的亨廷顿氏病(Huntington’sdisease,HD)亚急性动物模型的作用。方法通过对24只SD大鼠连续给药和间断给药方式进行比较,连续给药组3-NP 15 mg/kg/d连续5 d腹腔注射;间断给药组给予3-NP 15 mg/kg/d腹腔连续注射2 d后,间隔2 d,再连续注射3 d。结果改良的间断给药方式可明显降低动物死亡率(25%);并且通过Fluro-Jade B染色,可迅速特异地检测到纹状体变性的神经元。结论三硝基丙酸间断给药方式可有效建立HD动物模型。

关键词: 三硝基丙酸, 亨廷顿氏病

Abstract: Objective To evaluate the efficiency of two different administrations of 3-Nitropropionic acid(3-NPA) for inducing rat model of Huntington's disease.Methods Twelve SD rats were divided into two groups,one receiving continuous administration of 3-NPA(15 mg/kg daily,ip,for 5 days) and the other receiving intermittent administration(15 mg/kg daily,ip,for 2 days,no treatment for 2 days,and then 15 mg/kg daily,ip,for 3 days).Results Mortality rate was decreased to 25% and the degenerating cell bodies and affected fibers in striatum were found rapidly by Fluro-Jade B staining in the intermittent administration group.Conclusion The intermittent administration of 3-NPA is an effective method for inducing animal model of Huntington's disease.

Key words: 3-Nitropropionic acid, Huntington's disease, Animal model

李峰;徐群渊;. 三硝基丙酸诱导大鼠亨廷顿氏病模型的改进研究[J]. 实验动物科学.

LI Feng,XU Qun-yuan. Study of the Rat Model of Huntington's Disease Induced by 3-Nitropropionic Acid[J]. laboratory animal science.