关键词: 2型糖尿病, 高脂血症, 大鼠模型, 二甲双胍, 链脲佐菌素, 蔗糖水

Abstract: Objective To establish a Rat model of Type 2 diabetes induced by streptozotocin supplemented with 5% sucrose and high-fat diet,and to observe the therapeutic effects of metformin.Methods SD rats were injected peritoneally with streptozotocin(30 mg/kg) and 5% sucrose and high-fat diet were given to the rats for 68 days,and were engaged in glucose tolerance test.Blood samples were taken to detect glucose,blood lipid,sarum insulin and anti-oxidation level.Results Compared with the control group,the glucose,tolerance test after 2h glucose,glycated hemoglobin,serum insulin and insulin resistance index in high-fat feeds group were significantly higher(P<0.01).The insulin sensitivity index was significantly lower(P<0.01).TG,TC and free fatty acids(FFA) were significantly higher(P<0.05).The SOD activity decreased significantly(P<0.05).MDA was significantly increased(P<0.01).TC,FFA,glucose,glucose tolerance Anomaly,glycated hemoglobin,serum insulin and insulin resistance index,MDA In metformin-treated group were significantly lower(P<0.05).The insulin sensitivity index and SOD increased significantly(P<0.05).Conclusion 5% sugar water can improve the symptoms of anorexia,and combined with the low-dose streptozotocin and high-fat diet haS successfully induced a type 2 diabetes rat model with hyperlipidemia.Metformin has a hypoglycemic,lipid-lowering,anti-oxidation effects,and can improve insulin resistance,So it can be used as a positive therapeutic drugs to treat type 2 diabetes rat model with hyperlipidemia.

Key words: Type 2 diabetes mellitus, Hyperlipidemia, Rat model, Metformin, Streptozotocin