SPF家兔的培育及植入肠道正常菌群后生长性能的观察

实验动物科学

SPF家兔的培育及植入肠道正常菌群后生长性能的观察

张阔;王荫槐;周淑佩;康爱君;田枫;郑振辉;

北京大学医学部实验动物科学部,中国医学科学院实验动物研究所,北京大学医学部实验动物科学部,北京大学医学部实验动物科学部,北京大学医学部实验动物科学部,北京大学医学部实验动物科学部北京100083,北京100021,北京100083,北京100083,北京100083,北京100083

收稿日期:2006-03-25 修回日期:2006-03-25 出版日期:2006-03-25 发布日期:2006-03-25

Breeding of the SPF Rabbits and Observations of Their Growing Characteristics After Giveing Normal Intestinal Microflora

ZHANG Kuo~1,WANG Yin-huai~2,ZHOU Shu-pei~1,KANG Ai-jun~1,TIAN Feng~1,ZHENG Zhen-hui~1

(1.Department of Laboratory Animal Science Health Science Center,Peking University,Beijing 100083,China)(2.Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences, Beijing 100021,China

Received:2006-03-25 Revised:2006-03-25 Online:2006-03-25 Published:2006-03-25

摘要/Abstract

摘要： 目的对普通级实验家兔进行剖腹净化建立SPF家兔种群,提高实验用兔的质量,满足科研、生产及检定工作需要。方法采用剖腹摘取子宫术,在无菌隔离器内以人工哺乳方法将仔兔培育成无菌兔,再将双岐杆菌等肠道正常菌接种到无菌兔体内,使其成为SPF兔;对SPF环境中植入肠道正常菌兔和未植菌兔离乳后生长性能进行观察比较。结果人工培育出的SPF兔,经微生物和寄生虫检测,符合国家SPF兔标准;植入肠道正常菌群兔体重与未植入肠道正常菌兔生长体重相比极显著增加。结论通过剖腹摘取子宫术,人工哺乳仔兔和植入肠道正常菌群的方法建立了SPF实验用兔种群。

关键词: 摘取子宫术, 人工哺乳, SPF兔, 植菌

Abstract: Objective To establish the SPF rabbits breeding stock for our department,and to meet the needs of scientific research,production and test.Methods The rabbits were cleaned by cesarotomy,the young rabbits were reared in isolators by artificial breast feed.The young rabbits weaned were given with normal intestinal bacteria,such as bifidobacterium and Fusobacterium,then transfered them into the barrier system.Growing characteristics are compared between rabbits given with normal intestines microflora and non-intestines microflora rabbits.Results They were accorded with the standard of SPF rabbits of China,with microorganism of the rabbits examined.The body weights of the former increases significantly more than the latter.Conclusions The SPF rabbit breeding stock was established by cesarotomy in our department.

Key words: Cesarean section, Breast feed, SPF Rabbits, Intestinal microflora

张阔;王荫槐;周淑佩;康爱君;田枫;郑振辉;. SPF家兔的培育及植入肠道正常菌群后生长性能的观察[J]. 实验动物科学.

ZHANG Kuo~1,WANG Yin-huai~2,ZHOU Shu-pei~1,KANG Ai-jun~1,TIAN Feng~1,ZHENG Zhen-hui~1. Breeding of the SPF Rabbits and Observations of Their Growing Characteristics After Giveing Normal Intestinal Microflora[J]. laboratory animal science.

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