关键词: 人肝癌, 裸鼠, AFP, HBsAg

Abstract: The hetrotransplanted human hepatocellular carcinoma (HCC) secreting both AFP &-HBsAg was first established in Fujian province. The nude mice bearing tumor grew well and kept up 5th passage. Under histological examination, supported by electron microscopy and immunohistochemistry, the transplanted tumor retained approximately the morphological characteristics of original tumor. Shown by TEM, the transplanted tumor cells displayed some figures seen in normal liver cells, such as bile canilicull, junctionai complexes which were incomplete. HBsAg-like crystal structure was seen within RER and SER in some tumor cells. It is suggested that the close relationship between HBV and HCC be presented.It is reasonable that the development of double marked tumor in nude mice is an-useful animal model for the studies of cell biology, chemosensitivity and immunotherapy in HCC. A transfer solution, which is suitable for the survival of tumor cells, was presented in this paper. Owing to the limited passages of nude mice, the further efforts will andoubtedly be directed toward the schedule of biochemistry, growth dynamics and chromosome characteristics in hetrotransplanted HCC.

Key words: Human hepatocellular carcinoma, Nude mice, AFP ＆ HBsAg