关键词: 人食管癌, 移植癌模型, 裸鼠, 外科标本移植

Abstract: We have successfully established two human esophageal carcinoma models in nude mice by transplantation of surgical specimen from patients with esophageal cancer Histopathological study revealed that one of these to be squamous call carcinoma (HEC_2) and the other adeno-squamous cell carcinoma (HEC_6). Light and electron microscopy showed that they were similar to that of the original human tumors. The transplanted tumors have been maintained for more than three years through more than 40 passages. The tumors grew steadily and fast. The rate of inoculation was 100%. Bleomycin A5 and 5-Fluorouracil (5-Fu) were used for treatment of HEC_6. Results showed that Bleomycin A5 exhibited a remarkable growth inhibition on the HEC_6. The inhibitory rates of two separate experiments were 56.5% and 87.8% respectively comparing with the control group (P<0.01). The inhibitory rates of 5-Fu were 6.5% and 36.6% respectively, there was no statistic difference compared to the control group. HEC_2 was treated by X-ray or X-ray combined with Hematoporphyrin derivative (HPD), the tumor inhibitory rates were 57.7% and 85.4% respectively The results showed that combination of HPD with orthovoltage X-ray irradiation exhibited enhanced effect, and the result was repeatble.

Key words: Human esophageal carcinoma, Transplantable models, Nude mice, Transplantation of surgical specimen