不同抗凝比例对 SD 大鼠凝血指标的影响

doi:10.3969/j.issn.1006-6179.2024.05.012

实验动物科学 ›› 2024, Vol. 41 ›› Issue (5): 67-71.DOI: 10.3969/j.issn.1006-6179.2024.05.012

不同抗凝比例对 SD 大鼠凝血指标的影响

1. 四川省疾病预防控制中心,成都 610041) ( 2. 四川省都江堰市疾控中心,成都 611830

收稿日期:2023-12-28 出版日期:2024-10-28 发布日期:2024-10-28
通讯作者: 罗玲( 1987—) ,女,主管技师,研究方向为临床检验,E-mail:963003799@ qq. com.
作者简介:郑薇( 1987—) ,女,副主任技师,研究方向为毒理检验,E-mail:423535712@ qq. com.

Effect of Different Anticoagulation Ratio on Blood Coagulation Index in SD Rats

( 1. Sichuan Center for Disease Control and Prevention, Chengdu 610041,China)

( 2. Dujiangyan Center for Disease Control and Prevention,Chengdu 611830,China)

Received:2023-12-28 Online:2024-10-28 Published:2024-10-28

摘要/Abstract

摘要：

目的探讨不同抗凝比例对 SD 大鼠凝血结果的影响。方法 SD 大鼠 80 只,雌雄各半。腹腔静脉采血,部

分进行血钙(Ca 2+ ) 、血细胞比容( HCT)和血小板( PLT)的检测,其余按照枸橼酸钠抗凝管 1 ∶ 2、1 ∶ 4、1 ∶ 6、1 ∶ 8 和

1 ∶ 10 的抗凝比例,进行凝血酶原时间( PT)和活化部分凝血活酶时间( APTT) 的检测,并分析结果。结果 SD 大

鼠的 Ca 2+和 HCT 与人类正常值相近,但 PLT 远大于人类 PLT 的正常值范围。 APTT 随着抗凝剂量增加时间延长,

各抗凝组间差异有统计学意义(P<0. 05) ,PT 随着抗凝剂量增加时间延长,各抗凝组与 1 ∶ 2 或 1 ∶ 4 组间差异有统

计学意义(P<0. 05) 。 APTT 在抗凝比 1 ∶ 8 ~ 1 ∶ 10 时凝固曲线基线期缩短,PT 在抗凝比 1 ∶ 2 ~ 1 ∶ 4 时凝固曲线有

异常波动。结论 SD 大鼠最优抗凝比应适当高于人类,在 1 ∶ 6 左右能降低不合格标本的检出率,给动物实验提

供更可靠的检测结果。

关键词:

SD 大鼠, 抗凝比例, 凝血酶原时间, 活化部分凝血活酶时间

Abstract:

Objective To explore the effect of different anticoagulant ratio on coagulation result in SD rats. Method

There were 80 SD rats, half male and half female. After abdominal venous blood collection. Half of them were tested for blood calcium (Ca 2+ ) ,hematocrit ( HCT) and platelets ( PLT) , the remaining rats were tested for prothrombin time ( PT) and activated partial thromboplastin time (APTT) according to the anticoagulant ratio of 1 ∶ 2, 1 ∶ 4, 1 ∶ 6, 1 ∶ 8 and 1 ∶ 10. Result The Ca 2+ and HCT values of SD rats were close to the normal values of human, but PLT was much larger than the

normal value range of human. The time of APTT were prolonged with the increase of anticoagulant dose

(P< 0. 05) , the time of PT were prolonged with the increase of anticoagulant dose, and the difference

between other anticoagulant groups and 1 ∶ 2 or 1 ∶ 4 groups was statistically significant (P<0. 05) . The

baseline of APTT were shortened when the anticoagulation ratio were 1 ∶ 8-1 ∶ 10, and the coagulation

curve of PT were abnormal when the anticoagulation ratio were 1 ∶ 2 - 1 ∶ 4. Conclusion The optimal

anticoagulation ratio of SD rats should be appropriately higher than that of human being, and it can

reduce the rate of unqualified sample detection at 1 ∶ 6. It can provide more reliable detection result for

animal experiments.

Key words:

SD rats, anticoagulation ratio, prothrombin time, activated partial thromboplastin time

中图分类号:

Q95-3

郑薇, 夏祺悦, 刘科亮, 罗玲.

不同抗凝比例对 SD 大鼠凝血指标的影响

[J]. 实验动物科学, 2024, 41(5): 67-71.

ZHENG Wei, XIA Qiyue, LIU Keliang, LUO Ling.

Effect of Different Anticoagulation Ratio on Blood Coagulation Index in SD Rats

[J]. Laboratory Animal Science, 2024, 41(5): 67-71.

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不同抗凝比例对 SD 大鼠凝血指标的影响

Effect of Different Anticoagulation Ratio on Blood Coagulation Index in SD Rats

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