Objective To explore the relationship between the pH value of drinking water and intestinal
micro-organisms of experimental animals, and evaluate its potential impact on metabolism in vivo. Method
Twenty-four SPF grade male adult C57BL / 6N mice aged 5 - 6 weeks were randomly divided into three
groups according to body weight: blank control group (Control) , acidification group (Acid) , alkalization
group (Alkali) , providing daily drinking water pH 6. 3, pH 2. 5 and pH 8. 7, and the animals continued
to drink freely for 4 months. Observe and record the appearance, physical signs, behavior and activities
of animals every day, and measure the weight and water intake every week, collect fresh mouse feces
after the experiment, and the sequence of V3 - V4 region was determined by 16S rDNA amplification
technique to compare the structure and diversity of intestinal microbial community. Result The species
composition of intestinal micro-organisms in each test group is significantly different, and the species in
each group are significantly different. The analysis of species at the two taxonomic levels of phylum and
genus showed that the abundance of Firmicutes and Roseburia in the Control group was significantly
higher than that in the treatment group, while the abundance of Verrucomicrobia, Barnesiella, Bacteroides
and Alloprevotella was significantly lower. Conclusion Long-term intake of drinking water with pH 2. 5
and pH 8. 7 did not affect the growth and development of mice, and promoted the colonization of
beneficial bacteria related to carbohydrate and lipid metabolism in mice.
