Abstract: ? Canine Reovirus type 3,designated Reo-3 AMMS,originally obtained from a dog with respiratory signs and diarrhea in China,was further confirmed by electron microscope with ultrathin sectioning and phosphotungstic acid negative staining methods.The Reo-3 virions composed of dense core and two layered capsids aggeregate to crystalline arrangement.The Reo-3 replicates in cytoplasm and releases from disrupted cells.It showed that bodies of different contents and in varying forms were seen in nucleus and cytoplasm.A large amount of filementous and tubular structures observed in cytoplasm were deemed to be relevant to viral replication.

Key words: Canine, Reovirus\ BHK-21 Cell\ Ultrastructural Features

摘要： 本实验采用免疫电镜负染、细胞超薄切片透射电镜观察 ,证实所分离的病毒为犬呼肠病毒。病毒粒子由致密的核心和包围着核心的双层衣壳组成 ,有的为空心衣壳 ,多呈晶格状排列 ;病毒在胞浆增殖 ,装配 ,通过细胞崩解释放 ;细胞浆内可见大量的微丝与微管结构 ,认为与病毒复制有关。胞浆与胞核中均可见到内容物不同 ,形态各异的包涵体

关键词: 犬, 呼肠病毒, BHK-21细胞, 超微结构