laboratory animal science

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The Study of In Vitro Fertilization in Wild Mice

WANG Feng\|shan, GUAN Tong, MU Rui\|dong, YU Guo\|de   

  1. (Tianjin Institute of Industrial Hygiene and Occupational Diseases, Tianjin 300204
  • Received:2000-12-25 Revised:2000-12-25 Online:2000-12-25 Published:2000-12-25

野生小鼠体外受精技术实验初探

王凤山,管彤,穆瑞东,于国德   

  1. 天津市劳动卫生职业病研究所!天津300204,天津市劳动卫生职业病研究所!天津300204,天津市劳动卫生职业病研究所!天津300204,天津市劳动卫生职业病研究所!天津300204

Abstract: The techniques of in vitro fertilizaton and oviduct embryo transfer in mice were used to C\-\{57\}BL/6J and BDF\-1 mice. These techniques were also applied to the TW wild mice being inbred. The results of superovulation of female mice, fertilization of eggs and the quality of sperm taken from the epididymis of the males were observed and compared with those other international works. The differences of mechanism of in vitro fertilization between laboratory mice and wild ones were also discussed.

Key words: Wild mice, In vitro fertilization, Oviduct embryo transfer@

摘要: 应用实验小鼠体外受精技术对C57BL/6J小鼠和C57BL/6J与DBA/2杂交的F1 代BDF1 小鼠进行了体外受精实验研究 ,就体外受精相关指标与国际常规工作做了比较 ;同时将这些技术应用于实验动物化中的TW(TianjinWild)野生小鼠 ,就小鼠精子质量、野生小鼠体外受精情况及体外受精机理做了初步探讨

关键词: 野生小鼠, 体外受精, 输卵管移植