Abstract: Objective: To compare and research the protein composition of H 22 cell lines from different institutes.Methods: H 22 cell lines attained from three institutes were kept in abdominal cavity of KM mouse.The protein composition of different H 22 cell lines were compared with SDS polyacrylamide gel electrophoresis (SDS\|PAGE) and immunohistochemistry methods.Results: SDS\|PAGE electrophoresis showed that the numbers of protein bands of H 22 from different institutes were different,and there were five bands that their pigmentary intensities markedly varied,which indicated that the corresponding protein contents in different H 22 cell lines were different. The protein composition of PDGF\|A,TrkA,Gai,CDk2,NFκ Bp65 showed qualitative difference ( p <0.01).Conclusion: There were obvious differences among the different H 22 cell lines in the protein composition.

Key words: Murine Hepatoma 22, SDS\|PAGE, immunohistochemistry

摘要： 从三个单位引进小鼠肝癌H2 2 瘤株 ,昆明小鼠腹腔传代 ,制备瘤细胞 ,用SDS聚丙烯酰胺凝胶电泳 (SDS -PAGE)和免疫组化法 ,比较研究小鼠肝癌H2 2 瘤株细胞蛋白质表达的异同。结果发现三单位H2 2 瘤株细胞的电泳条带数和染色强度不同 ,其中有五个条带染色强度差异明显 ,提示相应蛋白质含量有明显不同 ;免疫组化染色测定H2 2 瘤株细胞PDGF -A、TrkA、Gai、CDk2、NFκBp6 5蛋白表达 ,其中CDK2阳性率两两单位间无显著差异 ,Gai阳性率两两单位间均有差异显著 (p <0 .0 1) ,PDGF -A、TrkA、NFκBp6 5阳性率反应不一。可见三单位H2 2 瘤株细胞蛋白质组成明显不同

关键词: 肝癌H_(22), SDS聚丙烯酰胺凝胶电泳, 免疫组化