Abstract: Objective To delve into the changes of immunologic functions in normatively developing of deficiency of QI of the spleen in rats.Method\ Based on improving and perfecting of the previous method of developing deficiency of qi of the spleen and diagnostic criteria for deficiency of \%qi\% of the spleen,the changes in partial cytoimmunity,nonspecific immunity,and erythrocyte immunity during developing deficiency of \%qi\% of the spleen by the preliminarily normative method were observed in Waster rats and the results were compared with those of the other five representative methods of developing deficiency of \%qi\% of the spleen.In preliminarily normative method of developing deficiency of \%qi\% of the spleen, purging by using drugs of bitter in taste and cold in nature,and wasting \%qi\%,dispelling the stagnated \%qi\% and checking upward perverted flow of \%qi\% with the durgs were adopted based on screening of the rat's constitution of traditional Chinese medicine,and oral administration of decoction of Sheng Da Huang (生大黄 Radix et Rhizoma Rhei),oral administration of decoction of the durgs for wasting \%qi\% and dispelling the stagnated \%qi\%,controlled feeding, overfatigue,and hypodermic injection of reserpine were adopted respectively in the other five representative methods.Results\ According to the changes in wet weight and index of spleen,thickness of thymic cortex,diameter of lymph sheath surrounding central artery of spleen,proliferative function of splenic T\|lymphocyte,killing rate of splenic NK cell against YAC\|1cell,and phagocytic activity of splenic macrophage,deficiency of \%qi\% of the spleen caused by the preliminarily normative method was considerably superior in conformity with the diagnostic criteria for deficiency of \%qi\% of the spleen to deficiency of \%qi\% of the spleen caused by the other five representative methods,and the restoration of deficiency of \%qi\% of the spleen caused by the preliminarily normative method was slower.Conclusion\ The goal of the optimization and normalization in developing of deficiency of \%qi\% of the spleen was attained in the experiment.

Key words: Syndrome of deficiency of qi of the spleen, Animal model, Normalization, Cytoimmunity, Nonspecific immunity, Erythrocyte immunity

摘要： 目的　在脾气虚证动物模型规范化的初步研究中 ,探讨其部分免疫功能改变。方法　首先改进、完善脾气虚证模型的诊断标准和造模方法 ,然后观察其部分细胞免疫、非特异性免疫、红细胞免疫功能改变 ;同时与 5种目前有代表性的脾气 (阳 )虚证模型进行比较。动物采用WistarⅡ级大鼠 ,雄性 ,分为对照组、初步规范组、大黄组、耗气破气组、限量营养组、劳倦过度组和利血平组。初步规范组采用体质、苦寒泻下加耗气破气降气法造模 ,其他模型组采用相应常规方法造模。取样检测指标前进行游泳或寒冷负荷。结果　从胸腺湿重和指数 ,胸腺皮质厚度 ,脾脏中央动脉周围淋巴鞘直径 ,脾脏T淋巴细胞增殖功能 ,脾脏NK细胞对YAC 1肿瘤细胞杀伤率 ,脾脏巨噬细胞中性红吞噬活性等指标的改变看 ,初步规范组的造模结果与脾气虚证模型诊断标准有较高的符合率 ,包括自然恢复较慢 ,且与诊断标准的符合率明显优于目前 5种有代表性的脾气 (阳 )虚证模型。结论　本实验达到了初步优化、规范脾气虚证模型的目的。

关键词: 脾气虚证, 动物模型, 规范化, 细胞免疫, 非特异性免疫