Abstract: Objective To compare the intravenous glucose tolerance test(IVGTT),serum glucose,serum insulin and plasma HbA1c in three different minipig strains.Methods Four month old Guangxi Bama minipigs and Guizhou minipigs,and five month old Wuzhi Shan minipigs were conducted with intravenous glucose tolerance test,and their serum glucose was detected by routine biochemical methods,serum insulin was detected by chemiluminescence,and HbAlc was detected by radioimmunoassay(RIA).All animals were kept under the same conditions during the test.Results In IVGTT test,serum glucose reached peak concentrations after 10 minutes of the glucose injection,and it fall to base level at 60 and 90 minutes in Guizhou minipigs and Bama minipigs respectively.Serum glucose in Wuzhi Shan minipigs fall to the base level at 120 minutes,which was higher than that of Guangxi BaMa minipigs and Guizhou minipigs at the time points during 30 minutes to 90 minutes(P<0.05).Among the three strains the value of HbAlc were higher in Guizhou minipigs(P>0.05),and lower in Wuzhi Shan minipigs (P<0.01).There were no significant differences of blood glucose concentration and insulin sensitivity among the three strains(P>0.05).Conclusion The serum glucose and related parameters were similar among three minipig strains.And the expression were similar too in IVGTT with a little difference in Wuzhi Shan minipigs.

Key words: Minipig, IVGTT, Serum glucose, Insulin, HbAlc

摘要： 目的比较三个品系小型猪静脉葡萄糖耐量及血糖相关指标的差异。方法实验用4月龄广西巴马小型猪和贵州小型猪,5月龄五指山猪,在相同饲养条件下进行实验。静脉注射50%葡萄糖进行静脉葡萄糖耐量试验;常规生化方法检测血清血糖;化学发光法检测血清胰岛素;放免法测定HbAlc。结果静脉葡萄糖耐量试验(IVGTT)中,注糖后10 min血糖达到峰值,贵州小型猪和广西巴马小型猪分别在60 min和90 min时恢复至实验前血糖值。五指山猪在120 min时恢复至注糖前水平,而且在30~90 min各时间点的血糖值高于广西巴马小型猪和贵州小型猪(P<0.05)。三组动物中,贵州小型猪HbA1c含量较高(P>0.05),五指山猪胰岛素水平较低(P<0.01)。三个品系小型猪的空腹血糖和胰岛素敏感指数差异不显著(P>0.05)。结论在IVGTT实验中,三个品系小型猪的血糖下降趋势基本一致,五指山猪有一定特殊性;相关血清生化指标测定值接近。

关键词: 小型猪, IVGTT, 血糖, 胰岛素, HbAlc