laboratory animal science

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Effect of Dietary Calcium,Phosphorus and Calcium∶Phosphorus Ratio on The Contents of The Microelements in Rats' Hepatic Tissue

AN Xing-lan,FU Qiang,ZHAO Shuang,WANG Zhi-hong,LIU Yuan~*   

  1. (Laboratory Animal Center of Academy of Military Medical Sciences,Beijing 100071,China
  • Received:2007-08-28 Revised:2007-08-28 Online:2007-08-28 Published:2007-08-28

日粮钙磷含量及比例对大鼠肝组织微量元素的影响

安星兰;付强;赵爽;王志红;刘源;   

  1. 军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心 北京100071,北京100071,北京100071,北京100071,北京100071

Abstract: Objective To observe the effect of dietary calcium,phosphorus and calcium∶phosphorus ratio on the contents of the microelements in rats' hepatic tissue.Methods 70 male Wistar rats aged 30 d were divided into 7 groups randomly according to their body weigh,10 for each group was considered as one batch for test.Rats in 7 groups were raised on diets with different dietary calcium and phosphorus in 42 d.Results In the same dietary phosphorus which had different calcium∶phosphorus ratio,the contents of Cu and Mn in hepatic tissue had no significant deviation(P>0.05),the content of Fe significantly decreased with the elevating of dietary calcium(P<0.05),and the contents of Zn and Mg had significant deviation between the groups of low calcium and high calcium(P<0.05).In the same dietary phosphorus which had different calcium∶phosphorus ratio,the content of Cu in the group of low dietary phosphorus was significant higher than the other groups(P<0.05),and the contents of Fe and Mn had significant deviation(P<0.05).In the same dietary calcium∶phosphorus ratio,the contents of Fe,Mn and Mg in the group of high dietary calcium and phosphorus was significant lower than the other groups(P<0.05).Conclusion In rats' hepatic tissue,dietary caclcium had effect on the contents of Fe,Zn and Mg,but had no effect on the contents of Cu and Mn;dietary phosphorus had effect on the contents of Cu,Fe and Mn,but had no effect on the contents of Zn and Mg.

Key words: Rat, Hepatic tissue, Microelements, Calcium, Phosphorus

摘要: 目的探讨钙磷含量及比例对大鼠肝组织微量元素含量的影响。方法将1月龄清洁级Wistar雄性大鼠随机分成7组,每组10只,饲喂7种不同钙磷含量的试粮,试验期42 d。结果相同磷进食水平下,肝铜、肝锰含量在不同钙磷比之间无显著性差异(P>0.05);肝铁含量随日粮钙水平的提高而显著下降(P<0.05);肝锌、肝镁含量在低钙组与高钙组之间存在显著性差异(P<0.05)。相同钙进食水平下,钙磷比4.0∶1组(低磷)的肝铜含量显著高于其它两组(P<0.05);肝铁、肝锰含量在三组之间存在显著性差异(P<0.05)。相同钙磷比水平下,高钙高磷组的肝铁、锰、镁含量明显低于其它组(P<0.0 5)。结论钙影响肝铁、锌、镁的含量,对铜、锰影响不明显。磷影响肝铜、铁、锰的含量,对锌、镁影响不明显。钙磷比为4.0∶1(Ca 1.00%,P 0.25%)时肝中微量元素含量最高,而钙磷比为1.2∶1(Ca 3.00%,P 2.50%)时肝中微量元素含量最低。

关键词: 大鼠, 肝组织, 钙, 磷, 微量元素