Abstract: In this study,total mRNA isolated from the ovary tissue of Guangxi Bama mini-pig were used as templates to synthesize cDNA by reverse transcription polymerase AMV.The partial sequence of activin β_A and β_B cDNA with length of 964 bp and 791 bp respectively,was obtained by PCR.The homology analysis showed that they are highly conservative.The homology rate was 99.79% and 99.62% respectively,compared with the activin β_A and β_B gene of other pig breeds reported in GenBank.Two nucleotides mutation were found in activin β_A gene,of which one mutation occurred in β_A mature peptide and the other in none coding part. Three nucleotides mutations occurred in the signal peptide of β_B,of which one is silent mutation.

Key words: Activin, Bama minipig

摘要： 目的通过克隆广西巴马小型猪活化素(activin)βA/βB基因cDNA,分析其序列,为研究活化素的生物学功能打下基础。方法提取广西巴马小型猪发情母猪的卵巢总RNA,并以其为模板,以下游特异性引物为反转录引物,在AMV酶作用下合成cDNA;用LA-Taq进行PCR扩增出包括成熟肽在内的activinβA及βB基因的部分序列,把两个序列分别连接到PMD-18载体进行克隆和序列分析。结果包括成熟肽在内的activinβA及βB基因的部分序列长度分别为964 bp和791 bp,两个亚基的序列比较保守,与GenBank上发表的猪activin两个亚基同源性达到99.79%和99.62%。activinβA基因有两个碱基发生突变,其中一个在成熟肽序列上,一个在非编码序列上;βB基因信号肽序列上有三个碱基发生突变,其中一个是同义突变。

关键词: 活化素, 巴马小型猪