Abstract: Objective To establish the hepatic fibrotic model of Guizhou minipig by multiplex factor.Methods Sixteen Guizhou minipig(8 females and 8 males) were divided into two groups including one control group and one experiment group at random.Control group was injected into peritoneum with olive oil and 0.9% NS,0.5 mL/kg twice a week,fibrosis model of experiment group was duplicated by peritoneal injection of 40% carbon tetrachloride and rabbit serum 0.5 mL/kg twice a week,for nine weeks.They were sacrificed at the end of the ninth weeks,then serum marker levels of liver function of ALT,AST,TP,ALB,A/G were determined.The liver were taken out and paraffin sections were stained by hematoxylin-eosin,Masson,Van Gieson,and were examined under light microscope(LM).The liver from experimental group were observed under electron microscopy(EM).Results Compared with the control group,serum ALT,AST activity of experiment group elevated significantly(P<0.01),ALB,A/G content decreased markedly(P<0.01);the pathological changes of liver of experiment group were observed under LM and EM.Conclusion\ Hepatic fibrosis model of Guizhou mini-pig was duplicated by peritoneal injection of 40% carbon tetrachloride and rabbit serum.

Key words: Guizhou mini-pig, Multiplex factor, Hepatic fibrosis, Model

摘要： 建立贵州小型猪复合因素所致的肝纤维化模型,为肝纤维化机制和治疗研究提供基础。方法贵州小型猪16头,雌雄各半,随机分为对照组和实验组。对照组每周两次给予溶剂橄榄油和生理盐水,实验组每周两次分别腹腔注射给予40%的四氯化碳(CCl4)橄榄油和兔血清各0.5 mL/kg,共9周,建立复合因素所致的肝纤维化模型。实验结束时,取血清查肝功ALT、AST、TP、ALB、A/G,取肝组织邻片做常规HE染色、Masson染色和Van Gieson染色,光镜和电镜下观察病理变化。结果染毒9周后,实验组血清ALT、AST比对照组明显增高(t值分别为0.0001、0.0004,P<0.01),ALB、A/G比对照组明显降低(t值分别为0.001、0.003,P<0.01);实验组肝组织光镜和电镜下观察病理切片均较对照组有明显的改变。结论复合因素CCl4、兔血清腹腔注射可导致贵州小型猪肝纤维化。

关键词: 贵州小型猪, 复合因素, 肝纤维化, 模型