Abstract: Objective To develop atherosclerotic model of apolipoprotein-E(ApoE) knockout mice.Methods Six male 8-week-old ApoE-knockout mice were fed with atherogenic diet for 8 weeks,and 6 male 8-week-old C57BL/6J mice were used as controls.The mice were sacrificed,and blood was drawn for determination of ssubjected to paraffin sections stained with hematoxylin and eosine to examine atherosclerotic lesions in aortic root.Results Serum TG,TC,and LDL-C levels in ApoE-knockout mice were significantly higher than those in C57BL/6J mice,whereas the reverse was true for serum HDL-C level.Lipid plaques of atherosclerosis were seen in the sections of aortic root of the ApoE-knockout mice.Conclusion Atherosclerotic plaques have already generated in 16-week-old ApoE-knockout mice,which may be ideal model for research on cardiovascular diseases.

Key words: ApoE-knockout mice, Arteriosclerosis, Blood lipid

摘要： 目的高脂饲养的雄性载脂蛋白E基因缺陷小鼠动脉粥样硬化模型的建立。方法选取8周龄雄性ApoE-/-小鼠,以C57BL/6J小鼠为对照组,高脂饲养8周后测定血清中总胆固醇、甘油三酯、高密度胆固醇脂蛋白、低密度胆固醇脂蛋白含量;小鼠心脏主动脉根部石蜡切片,常规HE染色,观察和测量动脉粥样硬化斑块的组织学特点及病变面积。结果ApoE-/-小鼠血清中总胆固醇、甘油三酯和低密度脂蛋白胆固醇的含量显著高于同年龄同性别C57BL/6J小鼠,高密度脂蛋白的含量显著低于同龄同性别C57BL/6J小鼠;高脂饲养下16周龄ApoE-/-小鼠主动脉根部切面可见明显的脂质斑块。结论在该浓度的高脂饲料饲养条件下,ApoE-/-小鼠在16周龄已形成脂质斑块,是研究动脉粥样硬化等心血管疾病较佳的动物模型。

关键词: 载脂蛋白E基因缺陷小鼠, 动脉粥样硬化, 血脂