Abstract: Objective The variation of lipid peroxides(LPO),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px) were tested in liver and kidney tissues of young and aged rats respectively;and to explore role of three enzymes in senescence process.Method There are ten twenty-one-day-SD rats and ten eighteen-month-SD rats.Liver and kidney tissues of rats were collected at 4 ℃;Thiobarbituric acid reaction was adopted to test LPO;Chemiluminescence used to test activity of SOD and activity of GSH-Px was tested through DTNB.Results Quantity of LPO in young rats' liver and kidney is significant lower compared to it in aged rats(P<0.01);Activity of SOD is markedly higher in young rat group than that in aged group(P<0.01);Activity of GSH-Px was obviously lower in young rat group contrast to aged group.Conclusion There were significant variation of LPO,SOD and GSH-Px in liver and kidney issues between young rats and aged rats.These variations are meaningful for study of senescence process.

Key words: Rats, Young, Aged, LPO, SOD, GSH-Px

摘要： 目的研究过氧化脂质(LPO)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)在幼龄和老龄SD大鼠肝、肾组织中的变化,探讨此三种酶在机体衰老过程中的意义。方法21日龄和18月龄SD大鼠各10只,4℃取鼠肝、肾组织,用硫代巴比妥酸法测定LPO的含量;化学发光法测定SOD活力;DTNB直接法测定GSH-Px活力。结果幼龄SD大鼠肝、肾组织中LPO含量明显低于老龄鼠(P<0.01);SOD活力显著高于老龄鼠(P<0.01);GSH-Px的活力明显低于老龄鼠(P<0.01)。结论LPO、SOD、GSH-Px在幼龄和老龄鼠中有显著的变化,对机体衰老过程的研究具有指标意义。

关键词: 大鼠, 幼龄, 老龄, LPO, SOD, GSH-Px