Abstract: Objective To prepare IBV agglutination antigen via treating with phospholipase C.Methods IBV was inoculated into allantoic cavity of 9-day-old SPF chicken embryo.The eggs were incubated at 37℃ for 48 hours.Allantoic fluid was collected and centrifuged at high speed for concentration,following to individually treated with trypsin and clostridium perfringen culture(containing phospholipase C).Then performing routine agglutination test and agglutination inhibition test.Results IBV haemagglutination showed strong non-specificity by means of trypsin treatment.On the contrary,IBV haemagglutination expressed powerful specific with phospholipase C because the haemagglutination could be inhibited by specific antiserum to IBV,simultaneously could not be inhibited by antiserum to ILTV,NDV,Adeno-1 and 3,APIV,AIV,IBDV,HP,MS and MG.Conclusion IBV treated with phospholipase C could be used for haemagglutination inhibition test.

Key words: Infectious Bronchitis Virus(IBV), Haemagglutination antigen

摘要： 目的利用磷脂酶C处理,制备传染性支气管炎病毒(IBV)血凝抗原。方法将IBV接种9日龄SPF鸡胚,48 h后收获尿囊液,经过高速离心浓缩,利用胰酶、产气荚膜梭状芽孢杆菌培养液(含磷脂酶C)处理后,进行常规血凝和血凝抑制试验。结果经过胰酶处理的IBV具有非常强的非特异性。而经过磷脂酶C处理的IBV,该凝集作用可被特异的IBV抗血清所抑制,而不能被ILTV、NDV、Adeno-1和Adeno-3,APIV、AIVI、BDV、HP、MS和MG等抗血清所抑制。结论磷脂酶C处理的IBV,可以用于血凝抑制试验。

关键词: 传染性支气管炎病毒, 血凝抗原