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GAO Fu-yun,GUO Jian,JIN Yao-ying, KONG Jing,ZHANG Yun,LIU Guo-ling,CHI Yong-chun
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高福云;郭建;靳耀英;孔晶;张韫;刘国玲;迟永春;
Abstract: Objective To study the difference of haematopoietic function between rats of different months-old age,especially the aged and young rats.Method Bone marrow haematopoietic ancestral cells were cultured in the monolayer soft agar of the lung cell culturing conditioned medium containing 15% colony stimulating factor(GM-CSF.).The cell colonies of karyocytes per 4×10~5 were calculated.Result There were 92.5±23.8 colonies in the young rats of 2.5 month-old,but 23.1±9.6 colonies in the aging rats of 24 month-old.As the GM-CFU of the 2.5month-old rats was considered as 100%,it was 37.0% of the 12month rats,35.7%of the 18 month old rats,and 27.2 of the 24month old rats.Conclusion The haematopoietic function of rats decreased as the age was increased.
Key words: Aging rat, Bone marrow haematopoietic function, Haematopoietic ancestral cell
摘要: 目的观察老年大鼠与青年大鼠造血功能的差别。方法采用含有15%刺激因子(GM-CSF)的大鼠肺条件培养基进行单层软琼脂法培养大鼠骨髓造血祖细胞,进行定量观察。结果肺条件培养基内刺激因子(GM-CSF)浓度为15%时活性最好。2.5月龄大鼠骨髓,每4×105个有核细胞中形成细胞集落(GM-CFU)为92.5±23.8,而24月龄老年大鼠则为23.1±9.6。以2.5月龄青年大鼠GM-CFU为100%,12月龄大鼠为37%,18月龄大鼠为35.7%,24龄大鼠则为27.2%。结论骨髓造血功能随月龄的增加逐渐减弱,老龄大鼠骨髓造血功能明显下降。
关键词: 老年大鼠, 骨髓造血功能
GAO Fu-yun,GUO Jian,JIN Yao-ying, KONG Jing,ZHANG Yun,LIU Guo-ling,CHI Yong-chun. The Study of Haematogenesis Function of Aging Rats[J]. laboratory animal science.
高福云;郭建;靳耀英;孔晶;张韫;刘国玲;迟永春;. 不同月龄实验大鼠造血细胞的定量观察[J]. 实验动物科学.
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