Abstract: Objective To analyze single nucleotide polymorphism(SNPs) of peroxisome proliferative activated receptor-γ coactivator 1 gene exon 8 in three strains of Chinese native minipigs(Bama minipigs,Wuzhishan minipigs,and Nongda minipigs) to provide basic data for their use in the researches of diabetes mellitus and metabolic diseases.Methods Genomic DNA was extracted from the blood samples of the minipigs,and served as templates for target gene segment amplified and selected by polymerase chain reaction (PCR).The PCR products were sequenced and analyzed for documenting single-nucleotide polymorphisms(SNPs).Results Five SNPs were found in the target region,Ala354Val(450C→T),Gly363Gly(478C→T),Arg369Arg(494C→A),Leu570Leu(913G→A),Ser551Ser(1039A→G),but only the variation of 450C→T to cause an encoding change Ala354Val.Conclusion There are variations on the SNPs in PGC-1 gene exon 8 among the different strains and individuals of native minipigs.

Key words: Minipig, PGC-1 gene, SNP_S

摘要： 目的分析我国特有的三个小型猪品系:巴马小型猪、中国农大小型猪、五指山小型猪过氧化物酶体增殖物激活受体γ协同激活子1基因第8外显子的单核苷酸多态性(SNPs)分布特点,为我国小型猪在糖尿病和代谢性疾病研究中提供基础资料。方法以三个品系小型猪基因组DNA为模板,应用特异性引物进行PCR扩增,扩增产物纯化测序,进行BLAST比对分析。结果测序结果表明在小型猪PGC-1基因外显子8有5个单核苷酸多态性位点为:Ala354Val(450C→T),Gly363Gly(478C→T),Arg369Arg(494C→A),Leu570Leu(913G→A),Ser551Ser(1039A→G),其中,只有45O位C→T的杂合突变导致了编码氨基酸的改变。结论小型猪品种不同PGC-1基因外显子8的多态性分布有很大差异。

关键词: 小型猪, PGC-1基因, 单核苷酸多态性