Abstract: Objective　Discuss the detailed steps and key considerations of the modified wire-bolus method in myocardial ischemia-reperfusion experiments in mice, with a view to improving the success rate and reducing the cost of the experiments. Methods　By describing in detail the operation steps such as preoperative preparation, anesthesia fixation, surgical incision, muscle separation, breaching the chest, extrusion of the heart, and cardiac ligation and summarizing the experience in the operation. Results This paper provides practical guidance for experimenters and the method of evaluating the success or failure of the model. Conclusion　The improved operation procedure is expected to provide reliable technical support for related research and advance the development of cardiovascular disease prevention and treatment research.

Key words: mice, myocardial ischemia-reperfusion, modified wire embolization method, animal model, empirical findings

摘要： 目的　探讨改良线栓法在小鼠心肌缺血再灌注实验中的详细步骤及关键注意事项,以期提高实验成功率并 降低成本。方法　通过详细描述手术前准备、麻醉固定、手术切口、肌肉分离、突破胸部、挤出心脏、心脏结扎等操 作步骤并总结操作中的经验。结果　本文为实验人员提供了实用的指导,并对模型成功与否的评估方法进行了介 绍。结论　改进后的操作流程有望为相关研究提供可靠的技术支持,推进心血管疾病防治研究的发展。

关键词: 小鼠, 心肌缺血再灌注, 线栓法改良, 动物模型, 经验总结