ObjectiveTo explore the detection methods for various indicators of biosafety independent ventilation cages ( IVCs) and evaluate the performance indicators of self-developed biosafety mouse IVCs.Methods According to the detection methods of RB / T199 - 2015 and DB23 / T 2057. 1 - 2017,various performance indicators of a certain imported biosafety type IVC ( IVC1) and a self-developed biosafety type IVC ( IVC2) were tested, and their safety was determined. Results All indicators of the prototype IVC and control IVC meet the requirements of national standards, the detection results of airflow velocity,air cleanliness, falling bacteria, noise, illumination,temperature and relative humidity are relatively consistent between the two IVCs.However, compared with IVC1, IVC2 had certain differences in pressure difference, ventilation frequency, and airtightness indicators. Conclusion The detection method has excellent operability and can be used for on-site inspection of biosafety mouse IVC. The self-developed biosafety mouse IVC has excellent performance, safety and reliability, and can be used for thebreeding of highly pathogenic animals in high-level biosafety laboratories.

biosafety, individually ventilated cages, physical indicators

目的 探讨生物安全型独立通风笼具 ( IVC) 的各项指标的检测方法,对自主研发的生物安全型小鼠 IVC 的性能指标进行评价。 方法 依据 RB / T199—2015 与 DB23 / T 2057. 1—2017 的检测方法,对某国外进口生物安全型IVC( IVC1)和自主研发的生物安全型 IVC( IVC2) 的各项性能指标进行检测,并判定其安全性。 结果 IVC1 与IVC2 的各项指标均满足相关标准的要求,两者在气流速度、空气洁净度、落下菌、噪声、照度、温度及相对湿度的检测结果较为一致,但是与 IVC1 相比,IVC2 在压差、换气次数、气密性指标存在一定的差距。 结论 检测方法操作性强,可用于生物安全型小鼠 IVC 的现场检验,自主研发的生物安全型小鼠 IVC 性能优良,安全可靠,可用于高等级生物安全实验室高致病性病原感染动物的饲养。

生物安全, 独立通风笼具, 性能检测