Abstract: Objective To explore the effect of specific immunity on inflammation in bronchoalveolarlavage fluid ( BALF) of asthmatic mice by analyzing Th1 / Th2 expression. Method Forty mice weredivided into control group, model group, SIT group, and dexamethasone group, with 10 mice in eachgroup. Except for the control group, asthma mouse models were established in the other groups. After thestimulation phase, the SIT group was injected with 1 mg of OVA, the dexamethasone group was given a0. 075 mg / mL solution, and the remaining rats were injected with an equal volume of PBS solution. Thelung function meter was used to detect airway responsiveness; the BALF inflammatory cells were observedby Wright-Giemsa staining; ELISA was used to detect cytokines IL-13, IL-4, IL-5, and INF-γ in BALF;HE staining was used to observe lung tissue morphology; PAS staining was used to observe cup-shapedcell activity in lung airways; and flow cytometry was used to detect the proportion of Th1 and Th2 in lungtissues. Result Specific immunity significantly reduced airway hyperreactivity in model mice, reducedinflammatory cell activity in BALF, and inhibited asthma occurrence and development by increasing Th1实验动物科学 41 卷while decreasing Th2. Conclusion Specific immunity can alleviate airway hyperresponsiveness andinhibit inflammation in asthmatic mice, improve cup-shaped cell proliferation, and relieve ventilation,possibly related to regulating Th1 / Th2 expression.

Key words:  bronchial asthma, specific immunity, inflammatory response, T-helper 1 cells, T-helper2 cells

摘要： 目的 基于 Th1 / Th2 表达探究特异性免疫对哮喘小鼠肺泡灌洗液炎性反应的影响。 方法 选择 40 只小鼠分为对照组、模型组、SIT 组及地塞米松组,每组 10 只。 除了对照组外均建立哮喘小鼠模型,激发阶段后 SIT 组注射 1 mg 的 OVA,地塞米松组灌胃 0. 075 mg / mL 溶液,其余大鼠注射等体积的 PBS 溶液。 采用肺功能仪检测气道反应;经瑞氏-吉姆萨染色观察肺泡灌洗液(BALF)炎性细胞;酶联免疫吸附法( ELISA) 检测 BALF 中细胞因子 IL-13、IL-4、IL-5 及 INF-γ;HE 染色观察肺组织形态;PAS 染色观察肺气道上皮杯状细胞活性;流式细胞术检测肺组织中Th1 及 Th2 占比。 结果 特异性免疫可显著降低模型小鼠气道高反应性、减少 BALF 内炎性细胞活性,并可通过升高 Th1 降低 Th2 而抑制哮喘发生发展。 结论 特异性免疫可缓解哮喘小鼠气道高反应性,抑制炎性反应,并通过改善气道上皮杯状细胞增生而缓解通气,可能与调节 Th1 / Th2 表达相关。

关键词: 支气管哮喘, 特异性免疫, 炎性反应, 辅助性 T 细胞 1, 辅助性 T 细胞 2