Abstract: Objective The type 2 diabetic zebrafish model were constructed and evaluated in order to systematically study the occurrence and development mechanism of type 2 diabetes mellitus ( T2DM) . Method In this study, the two groups of wild-type blue zebrafish were kept in the same environment with the same light, food, water and other conditions. The zebrafish in the experimental group were immersed in 2% glucose solution every other day for 28 days to establish the zebrafish model of type 2 diabetes. The establishment of the model was evaluated by observing the morphological changes, drawing the weight curve, and biochemical detection of glucose and lipid metabolism. Result After 28 days, the weight gain curve of zebrafish in experimental group was significantly higher than that in control group. During the induction, the zebrafish in the experimental group showed behavioral changes, they swam faster, were more sensitive to environmental stimuli, and shed white metabolites, while control zebrafish kept normal swimming speed and shed brown metabolites. The eyes of the experimental group showed a red and swollen appearance. Meanwhile,the tissue glucose level,the tissue triglyceride level and the tissue total cholesterol level in the experimental group was significantly higher than that in the control group (P<0. 05) . Conclusion The zebrafish model of type 2 diabetes mellitus can be successfully established after 28 days of glucose immersion every other day. The pathogenic status of zebrafish can be evaluated by behavioral observation, weight gain curve drawing, and glucose and lipid biochemical detection.

Key words: zebrafish, T2DM, animal model, glucose immersion method

摘要： 目的 为系统研究二型糖尿病( T2DM)的发生发展机制,构建二型糖尿病斑马鱼模型,并对模型构建情况进行评价。 方法 本研究采用葡萄糖浸泡法,将两组野生型蓝斑马鱼养在相同的环境中,光照、喂食、水源等条件一致,实验组斑马鱼隔天浸入 2%的葡萄糖溶液中,历时 28 d,构建斑马鱼二型糖尿病模型。 通过观察形态学变化、体质量曲线绘制以及糖脂代谢生化检测等指标来评价模型的构建情况。 结果 经过 28 d,实验组斑马鱼体质量增长曲线明显高于对照组。 在诱导过程中,实验组斑马鱼表现出游动速度加快、对环境刺激更敏感、排出白色代谢物等行为学改变,而对照组蓝斑马鱼保持正常游速且排出棕色代谢物。 实验组斑马鱼的眼睛呈现出红肿的外观。 同时,实验组斑马鱼组织葡萄糖水平、三酰甘油水平以及总胆固醇水平明显高于对照组( P< 0. 05) 。 结论 隔天葡萄糖浸泡法历经 28 d 可成功建立二型糖尿病斑马鱼模型,采用行为学观察、体质量增长曲线绘制和糖脂含量生化检测 3 种手段可以评价斑马鱼的疾病状态。

关键词: 斑马鱼, 二型糖尿病, 动物模型, 葡萄糖浸泡法