Abstract:In China, the incidence of Inflammatory Bowel Disease ( IBD) has increased significantly for the past few years. This disease is not easy to cure and comes with high expenditure. In order to find a cheap and efficient therapeutic method Illumina high-throughput sequencing techniques on mice with DSS-induced colitis. In this study, 15 C57BL / 6 mice were divided into the normal group, the model group and the Rb1 group. The treatment of model group and Rb1 group was 4 % ( m / v) DSS ( added in water) the first day, and 40 mg / kg ginsenoside Rb1 was fed to group Rb1 on the 2nd day. All mice were killed on the 9th day for samples of colon. The highthroughput sequencing platform Illumina was used to sequence the transcriptome of group normal, model, and Rb1. The Rb1 genes were compared with the model gene sets, and then the screened differential gene sets were analyzed by GO and KEGG pathway. The result showed that all indicators have improved after using Rb1. A total of 10,450 differentially expressed genes were GO annotated by Gene Ontology, containing 13. 5% molecular function, 68. 73% biological process, 17. 77% cellular component. Among the first 20 KEGG pathways that were most significantly enriched, the Neuroactive ligand-receptor interaction, the Calcium signaling pathway, and the cAMP signaling pathway were closely related to Rb1. Based on the result, we came to the conclusion that ginsenoside RB1 can alleviate the symptoms of DSS-induced colitis in mice. This work constructed the transcriptome sequence database of Rb1 on DSS-induced colitis in mice, which provides the sequence basis for future research on the gene mining function and metabolic pathways of ginsenoside Rb1.

摘要:目的 研究人参皂苷 Rb1 干预葡聚糖硫酸钠盐( Dextran Sulfate Sodium Salt,DSS)诱导结肠炎模型小鼠转录组学信息特征。 方法 C57BL / 6 雄性小鼠构建动物模型,将 15 只小鼠分为正常组、模型组和 Rb1 组,每组 5 只。 模型组和 Rb1 组给予 4% DSS 并自由饮水,第 2 天给 Rb1 组 40 mg / kg Rb1 进行干预。 持续 9 d,处死小鼠后,取结肠组织。 利用 Illumina 高通量测序平台分别对三组小鼠结肠组织进行转录组测序。 利用测定的转录组学数据对 Rb1 组和模型组两两比较之后进行重叠分析,筛选差异基因进行 GO 和 KEGG 分析。 结果 Rb1 干预后各项指标均有改善;GO 富集后其有 10 450 个有效差异表达基因得到 GO 注释,其中分子功能占 13. 5% ,生物过程占 68. 73% ,细胞组成占 17. 77% ;富集最显著的前 20 个 KEGG 通路中与 Rb1 干预后密切相关的有钙离子信号通路、神经活动配体-受体相互作用通路以及 cAMP 信号通路。 结论 人参皂苷 Rb1 能够缓解 DSS 诱导结肠炎模型小鼠的症状。 通过构建 Rb1 干预后 DSS 诱导结肠炎模型小鼠结肠组织转录组序列数据库,为以后 Rb1 功能基因的挖掘和参与代谢途径提供数据支撑,为临床应用提供新的思路。