Abstract:

Abstract: Objective To evaluate the therapeutic effect of Bushen Qinggan Shupi method on SD rats with type 2 diabetes. Method Diabetic rats were divided into diabetic model control group ( control group, n = 8) , diabetic model Bushen Qinggan Shupi group ( experimental group, n = 8) . Rats in the experimental group were infused with traditional Chinese medicine prescription prepared with distilled water, while rats in control group and normal feeding group( blank group, n = 8) were infused with sterile water. Six months later, samples were collected for intestine. Result Bacterial flora, GLP-1, PYY body weight, fasting blood glucose ( FBG) , blood lipid, and insulin secretion index were analyzed. Compared with the blank group, Bifidobacteria and Lactobacilli in the intestines of rats in the experimental group decreased significantly, while Enterococcus faecalis and Escherichia coli increased significantly. The result of Western bolt showed that the content of GLP-1 in the intestine of diabetic ratswas significantly different from that of untreated rats. The result of ELISA showed that there was no significantchange in PYY content between the blank group and the diabetic model control group, but the PYY content of the blank group increased significantly after the treatment of invigorating the kidney, clearing the liver and dispersing the spleen. There were significant differences in blood glucose, blood lipid, HOMA-β and body weight of diabetic rats before and after intragastric administration of kidney tonifying liver dispersing spleen. Conclusion The method of strengthening kidney, clearing liver and dispersing spleen has a certain control effect on diabetes mellitus, the mechanism of which may be through the improvement of intestinal flora and the content of GLP-1, PYY content.

摘要：

摘要:目的 本研究拟评估补肾清肝疏脾法对 2 型糖尿病 SD 大鼠模型的治疗作用及其对 MAPK 信号通路的影响。 方法 选用 2 型糖尿病 SD 大鼠模型,分为模型对照组( 对照组,n = 8) 和补肾清肝疏脾法组( 实验组,n = 8) 。实验组大鼠采用蒸馏水制备的中药方剂,对照组和正常饲养组( 空白组,n = 8) 使用 无 菌 水,连 续 灌 胃 6 个 月,然后采集样品,进行肠道菌群、胰高血糖素样肽-1( GLP -1) 、酪酪 肽 ( PYY) 含 量、体 质 量、空 腹 血 糖 ( FBG) 、血 脂及胰岛素分泌指数的分析。 结果 对照组大鼠 肠 道 内 双 歧 杆 菌 和 乳 酸 杆 菌 显 著 降 低、而 粪 肠 球 菌 及 大 肠 杆菌有明显的增加。 使用补肾清肝疏 脾 法 对 大 鼠 进 行 治 疗 后, Western bolt 结 果 显 示,糖 尿 病 模 型 大 鼠 肠 道 的GLP -1 含量较治疗前有显 著 性 差 异。 ELISA 结 果 显 示,空 白 组 与 对 照 组 大 鼠 相 比, PYY 含 量 未 见 显 著 性 变化;而实验组大鼠其含量增加显 著。 实 验 组 大 鼠 的 血 糖、血 脂、 HOMA-β、体 质 量 与 对 照 组 相 比,在 3 个 月、6个月均有显著性差异。 结论 补肾清肝疏脾法可控制糖尿病的发展,其作用 机 制 可 能 是 通 过 改 善 肠 道 菌 群、GLP -1 和 PYY 含量进行的。