Effect of Ermu Powder on the Expression of FGF, CTGF and Collagen Ⅰ in Rat Pulmonary Fibrosis Model Induced by Bleomycin

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Abstract:

Abstract: Objective To study the therapeutic effect and mechanism of Ermu powder on pulmonary fibrosis model in rats. Method Fifty SPF SD rats were divided into sham operation group, model control group, Ermu powder low-dose group ( 1. 08 g crude drug / kg) , Ermu powder high-dose group ( 2. 16 g crude drug / kg) , prednisone Control group ( 5. 4 mg / kg) . After ether anesthesia, the rats were incised to open the neck skin to expose the trachea, and 0. 2 mL (50 mg / mL) of bleomycin solution was instilled into the trachea. The sham operation group was infused with an equal volume of normal saline. Three days after modeling, intragastric administration was started. The administration volume was 10 mL / kg. The sham operation group and model control group were given intragastric administration of equal volume of distilled water, intragastric administration once a day for 4 weeks. At the end of the experiment, the rats were anesthetized with ether, the lungs were weighed, and the lung index was calculated ( pulmonary index = lung mass / body mass × 100%) . The right middle lobe was taken and fixed with paraformaldehyde solution for HE staining and immunohistochemistry dyeing. Result Compared with the model control group, the lung index of Ermu powder low-dose group and Ermu powder high-dose group was significantly reduced (P<0. 01) . Compared with the model control group, the histological grade of HE staining in Ermu powder low-dose group and Ermu powder high-dose group was significantly reduced (P<0. 05 or P<0. 01) . Compared with the model control group, there was no significant difference in the expression of FGF in lung tissue cells of Ermu powder low-dose group and Ermu powder high-dose group (P>0. 05) . Compared with the model control group, the expression of CTGF in lung tissue cells of Ermu powder low-dose group and Ermu powder high-dose group was significantly reduced (P< 0. 01) . Compared with the model control group, the expression of Collagen Ⅰ in lung tissue cells of Ermu powder low-dose group and Ermu powder high-dose group was significantly reduced ( P < 0. 05) . Conclusion Ermu powder can reduce the inflammatory and fibrotic pathological grades of bleomycininduced pulmonary fibrosis model in rats. It may inhibit the expression of CTGF and Collagen Ⅰ in lung tissues to inhibit bleomycin-induced rat lung The role of fibrosis.

摘要： 摘要:目的研究二母散对大鼠肺纤维化模型的作用及其作用机制。方法 50 只 SPF 级 SD 大鼠按体质量随机分为假手术组( SC 组) 、模型对照组(M 组)、二母散低剂量组(1. 08 g 生药/ kg,EL 组)、二母散高剂量组(2. 16 g 生药/ kg,EH 组) 、泼尼松对照组(5. 4 mg / kg,PR 组) 。大鼠经乙醚麻醉后切开颈部皮肤,暴露气管,气管内滴注博莱霉素溶液 0. 2 mL(50 mg / mL) ,假手术组滴入等体积的生理盐水。造模 3 d 后开始灌胃给药,给药剂量为 10 mL / kg,假手术组和模型对照组灌胃等体积的蒸馏水, 1 次 / d,连续给药 4 周。实验结束时大鼠经乙醚麻醉,取肺脏称质量,计算肺脏指数(肺脏指数 = 肺质量 / 体质量×100% ) ,取右肺中叶,多聚甲醛溶液固定,用于 HE 染色和免疫组化染色。结果与模型对照组相比,二母散低剂量组肺脏指数、肺组织细胞 CTGF 表达显著降低( P< 0. 01) ,HE 染色病理分级和肺组织细胞 Collagen Ⅰ 表达明显降低( P< 0. 05) ;二母散高剂量组肺脏指数、HE 染色病理分级、肺组织细胞CTGF 显著降低( P<0. 01) ,肺组织细胞 Collagen Ⅰ 表达明显降低( P<0. 05) 。结论二母散可能通过抑制 CTGF 和Collagen Ⅰ 在肺组织的表达,进而降低肺纤维化模型肺组织炎症及纤维化病理级别,最终产生抑制博来霉素诱导大鼠肺纤维化形成的作用。

LI Xiang, LIU Chang. Effect of Ermu Powder on the Expression of FGF, CTGF and Collagen Ⅰ in Rat Pulmonary Fibrosis Model Induced by Bleomycin [J]. Laboratory Animal Science, 2021, 38(2): 1-.

李想, 刘畅. 二母散对博来霉素诱导大鼠肺纤维化模型FGF、CTGF 和 Collagen Ⅰ 表达的影响 [J]. 实验动物科学, 2021, 38(2): 1-.

Effect of Ermu Powder on the Expression of FGF, CTGF and Collagen Ⅰ in Rat Pulmonary Fibrosis Model Induced by Bleomycin

二母散对博来霉素诱导大鼠肺纤维化模型FGF、CTGF 和 Collagen Ⅰ 表达的影响

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