The Key Points of Genotype Identification in Genetically Modified Mice Model

Laboratory Animal Science ›› 2021, Vol. 38 ›› Issue (1): 74-.

( National Institutes for Food and Drug Control, Beijing 102696, China)

Online:2021-02-28 Published:2021-04-14

遗传修饰小鼠模型基因型鉴定技术要点介绍

(中国食品药品检定研究院实验动物资源研究所,北京 102696)

Abstract

Abstract:

Abstract:Recently, with the development of biomedical research and gene editing technology, there are more and more animal models have been established. Due to the different production methods, the genotype identification technology is also different, resulting genotype identification disordered phenomenon. This paper is based on the method of the genetically modified mice model,introduced the genotype identification key points of the random transgenic mice, conditional knockin mice, and knockout mice. Through this paper, we want to achieve standardize the genotype identification method and ensure the accuracy of animal experimental materials, so as to promote the application and development of animal models in basic and applied science and technology fields.

摘要：

摘要:近年来,随着生物医学研究及基因编辑技术的发展,出现了越来越多的动物模型。由于制作方法的不同,其基因型鉴定技术方法也不一样,导致基因型鉴定的工作存在混乱无序的现象。本文根据遗传修饰小鼠模型制作方法进行分类,介绍了随机转基因小鼠、条件性基因敲入小鼠、基因敲除小鼠三类常见模型的基因型鉴定技术要点,规范遗传修饰小鼠模型基因型鉴定方法,保证动物实验材料的准确性,从而推动动物模型在基础和应用科技领域的应用与发展。

LIU Susu, WANG Chenfei, YUE Bingfei, LI Guanming, FAN Changfa. The Key Points of Genotype Identification in Genetically Modified Mice Model[J]. Laboratory Animal Science, 2021, 38(1): 74-.

刘甦苏, 王辰飞, 岳秉飞, 李冠名, 范昌发. 遗传修饰小鼠模型基因型鉴定技术要点介绍[J]. 实验动物科学, 2021, 38(1): 74-.