Abstract: Objective The guinea pig colony was decontaminated by caesarean section. Genetic testing confirms that the decontaminated colony conforms to the genetic characteristics of the closed colony. Method Female and male individuals were widely selected in different breeding units in production groups, mated at 1 ∶ 1. Cesarean section at 70 days after mating. The offspring fed by artificial milk. Breeding F1 generation by cycle mating system. DNA were extracted from 30 individuals ear tissue samples in F1 generation, 18 guinea pig microsatellite markers were used to genetic testing. Result A total of 85 pups were delivered by Caesarean section, with 56 surviving and 47 weaning. Rate of surviving was 65. 9%, rate of weaning was 83. 9%. Eighteen microsatellites loci