Abstract: Objective To analyze the identification and breeding method of ifnar knockout mice, and provide an ideal animal model for virus further studying. Method The introduced homozygous ifnar gene knockout mice were bred and reproduced in one cage with one male and two female mice together. The offspring mice genomic DNA was extracted from mice tail and amplified by PCR, and PCR products were analyzed by agarose gel electrophoresis for genotype identification. Result The homozygous ifnar knockout mice were breeding successfully in our facility,and the genotype can be identified by our established PCR method .

Key words: Ifnar, knockout, mouse, breeding, genotype identification

摘要： 目的 对Ifnar基因敲除小鼠繁育及鉴定方法进行分析，为多种病毒研究提供理想的动物模型。方法 将引进的纯合Ifnar基因敲除小鼠，以1雄2雌的合笼方式进行饲养繁殖，从仔鼠中提取鼠尾基因组DNA，PCR法扩增目的基因片段，琼脂糖凝胶电泳进行基因型结果判定。结果 Ifnar基因敲除小鼠繁育成功，获得了一批基因敲除鼠，使用PCR方法成功鉴定出Ifnar基因敲除纯合子小鼠。

关键词: I型干扰素受体, 基因敲除, 小鼠, 繁育, 基因型鉴定