Abstract: Objective To genetic monitoring of Guangxi Macaca fascicularis from different breeding groups, and analyze their genetic background, provide basic information for establishing the genetic quality monitoring method and population repository of Guangxi Macaca fascicularis.Method Twenty microsatellite DNA markers and capillary electrophoresis were adopted to genetic detect Guangxi Macaca fascicularis from three different breeding groups, and the genetic variation parameters within and between populations were calculated.Result A total of 237 alleles were detected in the Guangxi Macaca fascicularis, the observed allelic number was ranging from 5 to 19, with a mean of 11.85. The mean expected heterozygosity was 0.85, and the mean polymorphism information content was 0.817. In the three different breeding groups(F,N and W), respectively, 158, 158 and 173 alleles were detected, the mean expected heterozygosity was 0.8371, 0.8318 and 0.8642, and the mean polymorphism information content was 0.7692, 0.7653 and 0.8001. The three breeding groups showed the high genetic diversity. The Hardy-Weinberg equilibrium test for 3 breeding groups showed that most loci were in H-W equilibrium. Respectively, the average Fis, Fit, Fst of all loci was 0.0235, 0.0628, 0.0402, the index of gene flow was 5.9616, which implied that 3 breeding groups was nearly under the random mating system and had low genetic differentiation. The genetic distance was ranging from 0.2556 to 0.3223, the genetic similarity was 0.7245 to 0.7745. Cluster analysis showed that F population first clustered with N population, after that clustered with W population, it is conformed to their historical introduction.Conclusion The study effectively analyzed the genetic diversity and genetic relationship of Guangxi Macaca fascicularis, and the 20 microsatellite DNA markers selected in this study could be used to detect genetic quality of Macaca fascicularis．

Key words: Macaca fascicularis, Microsatellite DNA markers, Genetic monitoring

摘要： 目的 对广西食蟹猴不同生产繁殖群进行遗传检测，分析广西不同繁殖群食蟹猴的遗传背景，为建立广西食蟹猴遗传质量检测方法和种群资源库提供基础资料。方法 应用20个微卫星基因位点和毛细管电泳技术对广西3个不同生产群食蟹猴进行遗传检测，并计算群体内和群体间的遗传变异参数。结果 共检测到等位基因237个，其观察等位基因数(Na)为5～19个，平均11.85个；平均期望杂合度(He)为0.85；平均多态信息含量(PIC)为0.817。3个不同生产群(F、N和W)分别检测到等位基因158、158和173个，平均期望杂合度(He)分别为0.8371、0.8318和0.8642；平均多态信息含量(PIC)分别为0.7692、0.7653和0.8001，3个不同生产群存在较高的遗传多态性。3个生产群Hardy-Weinberg平衡检验多数位点处于H-W平衡。群内近交系数Fis均值为0.0235，总群体近交系数Fit均值为0.0628，遗传分化系数Fst均值为0.0402，基因流Nm均值为5.9616，表明3个生产群基本处于随机交配状态，群体间遗传分化很小。3个生产群遗传距离为0.2556～0.3223，遗传相似度为0.7245～0.7745，聚类分析显示F群体和N群体先聚为一类，再和W群体聚为一类，符合3个繁殖猴场各自关联引种历史特征。结论 本研究有效分析了广西食蟹猴的遗传多态性和群体间的遗传关系，所选的20个微卫星基因位点可用于食蟹猴遗传质量检测。

关键词: 食蟹猴, 微卫星DNA标记, 遗传检测