Abstract: Objective To provide possible genetic markers for Labrador Retrievers and Golden Retrievers, 3 SNPs in canine hip dysplasia (CHD) related genes COL6A3 and FN1 were detected. Method Eight typical CHD in Labrador Retrievers and 5 typical CHD in Golden Retrievers confirmed by imaging diagnosis were collected from everywhere of China, 23 normal Labrador Retrievers and 7 normal Golden Retrievers were provided by China Guide Dog Training Centre. The vein blood was sampled and the genomic DNA was isolated from white blood cells. The PCR/sequencing method was used to analyze the variants of 2 SNPs in COL6A3 (CFA25: 51031100, 51040259), and 1 SNPs in FN1 (CFA37: 25095511). In addition, the association between the SNP variants and CHD was analyzed by Chi-square Test. Result The SNPs variants of COL6A3 (CFA25: 51031100, 51040259), and FN1 (CFA37: 25095511) were not significantly associated with CHD (P>0.05). Conclusion The SNPs of COL6A3(CFA25∶51031100, 51040259) and FN1 (CFA37: 25095511) cannot be used as a practical genetic detection index for CHD diagnostics in Labrador Retrievers and Golden Retrievers.

Key words: CHD, Labrador Retrievers, Golden Retrievers, COL6A3, FN1

摘要： 目的 检测拉布拉多犬与金毛寻回猎犬基因组DNA中与髋关节发育不良密切相关的COL6A3基因、FN1基因中的3个SNP位点，以期为拉布拉多犬与金毛寻回猎犬幼犬的选育提供有效的遗传学诊断方法。方法 全国范围内募集经过影像学诊断的髋关节发育不良的拉布拉多犬8只、金毛寻回猎犬5只，中国导盲犬大连培训基地提供经影像学诊断的健康的拉布拉多犬23只、金毛寻回猎犬7只作为实验对象，静脉采血，提取血细胞的基因组DNA样本，采用PCR/测序方法对COL6A3基因上的2个SNP位点(CFA25∶51031100，51040259)和FN1基因的1个SNP位点(CFA37∶25095511)进行基因型检测，并统计分析SNP位点的基因型与髋关节发育不良的关联度。结果 COL6A3(CFA25∶51031100，51040259)和FN1(CFA37∶25095511)SNP位点的基因型与犬的髋关节发育不良没有显著相关性(P>0.05)。结论 COL6A3(CFA25∶51031100，51040259)和FN1(CFA37∶25095511)SNP位点均不能成为拉布拉多犬与金毛寻回猎犬髋关节发育不良的实用性遗传学诊断指标。

关键词: 髋关节发育不良, 拉布拉多犬, 金毛寻回猎犬, COL6A3, FN1