Abstract: Objective In order to compare the difference between Carnoys and formaldehyde fixation solution on goblet cell in rat colon, we stained the colon specimens. Method The rat colon specimens of normal and constipation model rats were fixed with Carnoys or 4% formaldehyde fixative to prepare paraffin sections respectively. By means of staining with HE or PAS/Alcian Blue individually according to its protocol and counting the number and color intensity of goblet cells, we compare the difference between the two fixatives fixed specimens. Result The results indicate that the number of goblet cells fixed with Carnoys and the formaldehyde fixative group has no significantly different (P>0.05, n=10), while the color intensity of the goblet cell stained with PAS/Alcian Blue was notable enhancement compared with the formaldehyde fixative group(P0.05, n=10). Conclusion These results confirmed that the treatment with Carnoys fixative was superior to formaldehyde fixative in the morphological observation of goblet cells. In the preparation of different experiments and specimens, we should choose the fixative solution depend on the characteristics of the experimental study object.

Key words: Carnoys fixative, formaldehyde fixative, goblet cell, constipation model

摘要： 目的 通过对大鼠结肠切片标本染色对比卡诺液与甲醛固定液处理标本的区别。方法 将正常大鼠与便秘模型大鼠的结肠组织标本分别用卡诺液与甲醛固定液处理，制成石蜡切片标本后，用HE染液和高碘酸希尔法阿尔新蓝染液分别进行染色，统计杯状细胞的数量和着色度对比两种固定液所固定标本的区别。结果 与甲醛固定液相比，同组标本用卡诺液固定的杯状细胞数量无显著性差异(P>0.05,n=10)，着色度显著增强(P0.05，n=10)。结论 在杯状细胞的形态学观察中，用卡诺液处理标本明显优于甲醛固定液。在不同实验的标本制备中，固定液的选择应根据实验研究对象的特性而确定。

关键词: 卡诺液, 甲醛固定液, 杯状细胞, 便秘模型