Abstract: This paper is reviewed a identification method of the experimental research and the progress appraisal for miniature pig inbred line, including: skin allograft transplantation,physiological and biochemical marker，isozyme protein identification, DNA genetic marker, SNP analysis method and so on.The comprehensive analysis is show that the technology of allogeneic skin grafts is the best identification method for a large mammal inbred line, which is a scientific basis for development and utilization of innovation resources on pig inbred line in China. It will be provided the scientific testing technology and method for identification of large mammals inbred line and make our country is a international leading position in large mammals inbred line study also.

Key words: Wuzhishan miniature pig, inbred line, body skin graft, immune rejection

摘要： 本文综述了国内外小型猪近交系鉴定方法的研究进展，包括：异体皮肤移植鉴定、基因组水平鉴定、生理生化标记、同工酶蛋白质鉴定、DNA遗传标记、SNP分析等分子遗传学的方法。综合分析，异体皮肤移植技术是大型哺乳动物近交系最佳鉴定方法。该技术将为我国创新资源近交系猪培育和开发利用奠定科学基础，为大型哺乳动物近交系鉴定提供科学的检测技术和方法，并使我国大型哺乳动物近交系研究处于国际领先地位。

关键词: 五指山小型猪, 近交系, 异体皮肤移植, 免疫排斥反应