Abstract: Objective To study the effects of 137Cs irradiation on the HSP70 expression of livers and kidneys in laboratory red crucian carps and explore the radioprotective effect of ginseng decoction on laboratory red crucian carp. Method The laboratory red crucian carps were randomly divided into 8 groups: blank control group, ginseng control group, 4.1Gy irradiation group, 4.1Gy ginseng prevention group, 4.1Gy ginseng treatment group, 8.2Gy irradiation group, 8.2Gy ginseng prevention group and 8.2Gy ginseng treatment group. Laboratory red crucian carps were exposed to 137Cs irradiation, or given ginseng decoction for 7 days before irradiation treatment, or treated with ginseng decoction for 7 days after irradiation treatment. Then the livers and kidneys of laboratory red crucian carps were collected separately, and the expression of HSP70 in each group was detected by Western blot method . Result Compared with the blank control group, the expression of HSP70 protein was increased after 4.1Gy or 8.2Gy irradiation. Compared with the irradiation groups, the expression of HSP70 was lower after the prevention or treatment of ginseng decoction. There was no significant influence on HSP70 expression just by treatment with ginseng decoction.It was found that there were no obvious differences in HSP70 protein expression between ginseng prevention groups and ginseng treatment groups. Conclusion 137Cs irradiation can increase the HSP70 expression of livers and kidneys of the laboratory red crucian carps, while the expression of HSP70 is reduced after the prevention or treatment of ginseng decoction.

Key words: Ginseng Decoction, Laboratory Red Crucian Carp, 137Cs Irradiation, HSP70

摘要： 目的 研究137Cs辐射对实验红鲫肝肾组织HSP70表达的影响，并探讨人参煎剂对实验红鲫的辐射防护效应。方法 将实验红鲫随机分为8组，即空白对照组、人参对照组、4.1Gy辐照组、4.1Gy人参预防组、4.1Gy人参治疗组、8.2Gy辐照组、8.2Gy人参预防组及8.2Gy人参治疗组。分别以137Cs辐照处理，或辐照处理前以人参煎剂浸浴7 d做预防处理，或辐照处理后以人参煎剂浸浴7 d做治疗处理，然后分别采集实验红鲫肝脏和肾脏组织，利用Western blot方法检测各组HSP70表达量。结果 与空白对照组相比，经4.1Gy或8.2Gy辐照处理后的HSP70蛋白表达量均增加；与辐照组相比，经人参煎剂预防或治疗处理后的HSP70表达量均下降；单独的人参煎剂处理，对HSP70蛋白表达量无明显影响；比较人参煎剂做预防与治疗处理，发现两者之间的HSP70蛋白表达量差异不明显。结论 137Cs辐射能够引起实验红鲫肝脏和肾脏的HSP70表达量升高，再经人参煎剂预防或治疗处理后的HSP70表达量均下降。

关键词: 人参煎剂, 实验红鲫, 137Cs辐射, 热休克蛋白70