Abstract: Objective Establish specific pathogen-free Anas platyrhyncha var.domestica breed populations. Method Highly quality breed species of Shaoxing Shedldrake No. I and Jinding duck breed eggs were based on as original populations, various biological purification measures were adopted, including eliminated specific avian diseases antibodies and antigen positive individuals, immunized with killed vaccines, raised in positive pressure high efficiency particulate air filter, fed with 60Co-treated feed, acidified water, strictly controlled the flow of people and logistics. Simultaneously, tests of molecular genetics, biological characteristics and disease susceptibility were carried out to standardize the ducks. Result HBK-SPF and SPF-JD duck breed populations with specific pathogens-free, clear genetics background and disease susceptible were successfully established. Conclusion The established specific pathogen-free duck breeds will enrich Chinese laboratory animals resources and provide important support for life science research and development of biological products.

Key words: Shedldrake ducks, Specific pathogen-free, Breed

摘要： 目的 建立无特定病原体麻鸭种群。方法 以优质培育品种绍兴麻鸭白壳I号和金定鸭种卵为基础，通过疫病监测淘汰抗体抗原阳性个体、灭活疫苗免疫、整个生命周期应用鸭正压隔离器饲养、60Co射线辐照饲料、酸化饮用水、严格控制人流、物流等净化措施，同时开展了多种分子遗传学检测、生物学特性数据测定和疫病敏感性试验，以实现实验动物化。结果 成功建立了无特定病原、遗传学背景清晰、疫病敏感的HBK-SPF和SPF-JD鸭种群。结论 SPF麻鸭种群丰富了我国实验动物资源，为生命科学研究和生物制品研发提供了重要条件支撑。

关键词: 麻鸭, 无特定病原体, 种群