Abstract: Abstract: Objective To investigate the changes of cytokine levels after the infection of rat glioma cells C6 by H-1 parvovirus． Method C6 cells were taken after infection H-1 parvovirus at different time points，while the normal C6 cells were taken in these time points，and the ELISA． Methods were used to detect the expression levels of TNFα，IL-6，TGF-β1 and IL-17A． Result The concentrations of TNF-α，TGF-β1 and IL-17A in C6 cells were increased during culture，and which of IL-6 was no significant change; The concentrations of TNF-α，TGF-β1 and IL-17A in H-1 parvovirus infected C6 cells were no significant change，while IL-6 concentration was significantly increased over time． Conclusion H-1 parvovirus infection leads to changes in C6 cytokine expression levels．

Key words: <p>H-1 parvovirus, rat Glioma cells C6, cytokine</p>

摘要： 摘要: 目的探讨H-1 细小病毒感染大鼠神经胶质瘤细胞C6 后细胞因子水平的变化。方法取感染C6 细胞后不同时间点的H-1 细小病毒，同时取相同时间点的正常C6 细胞，采用ELISA 方法检测同时检测TNFα、IL-6、TGF-β1和IL-17A 的表达水平。结果正常C6 细胞中TNF-α、TGF-β1 和IL-17A 的浓度随培养时间增长而提高， IL-6 浓度无明显变化; H-1 细小病毒感染的C6 细胞中TNF-α、TGF-β1 和IL-17A 浓度无明显变化，而IL-6 浓度随感染时间推移而显著增加。结论H-1 细小病毒感染导致C6 细胞中细胞因子表达水平的变化。

关键词: <, p>, H-1 细小病毒, C6 细胞, 细胞因子<, /p>