Abstract: Abstract: Clustered regularly interspaced short palindromic repeats ( CRISPR) system was a new genetic modification tool． Its advantages were short work time，low cost，high efficiency and so on． Here we review the CRISPR-Cas system structure，development history，classification，mechanism and application in biology research．

Key words: CRISPR, CRISPR-Cas system, biology research

摘要： 摘要: 成簇的规律间隔的短回文重复序列( clustered regularly interspaced short palindromic repeats，简称为 CRISPR) 系统是一种新的基因修饰技术，具有制作周期短、成本低和作用高效等优点。本文从结构、研究历史、分类和分布、作 用机制和其在生物学研究中的应用等方面介绍 CRISPR-Cas 系统。

关键词: CRISPR, CRISPR-Cas 系统, 生物学研究