Abstract: Objective\ Study on the expression of human insulin gene by baculovirus expression system in housefly. Method\ The recombinant baculovirous contained human insulin gene weas used to infect housefly larva, the human insulin was detected by Tricine\|SDS\|PAGE and Western\|blot. Result\ Human insulin was expressed by infected housefly larva. And the most expression level was 37.401μIU/ml. Conclusion\ Human insulin could be expressed in housefly larva with baculovirous expression system.

Key words: Baculovirus expression system, Human insulin, Housefly

摘要： 目的　利用杆状病毒表达系统进行人胰岛素基因在家蝇中表达的研究。方法　用携带有人胰岛素基因的重组杆状病毒感染家蝇幼虫 ,用放射免疫技术、Tricine SDS PAGE和Western blot技术对蝇蛆胰岛素表达水平进行检测。结果　重组的杆状病毒感染家蝇幼虫后 ,蝇蛆中有人胰岛素的表达 ,表达量为 37.4 0 1μIU mL ,占蝇蛆总蛋白的0 .5 83%。结论　利用杆状病毒表达系统可以在家蝇蝇蛆中表达人胰岛素

关键词: 杆状病毒表达系统, 人胰岛素基因, 家蝇