Abstract: The aim of this study was to development an in vitro test for the detection of pyrogen in order to replace the rabbit pyrogen test. Recently the THP 1 monocytic cells was assessed for tumor necross factor (TNF) α for the probability of the novel test. When THP 1lines is incubated in the presence of reference standard endotoxin, they secrete “endogenous pyrogen" TNF α determined by ELISA after a fixed incubation time (6 hours). These results show that the correlation coefficient is greater than or equal to 0.980 in the range of 0.32EU/mL 200EU/mL between the TNF α and the endotoxin. The same result has got from the other kind endotoxin with equal effect in the LAL/TAL test. Therefore we think this novel test will serve as an aternative pyrogen test.

Key words: Pyrogen, Pyrogen test, Cytokine, THP 1, TNF α

摘要： 为了寻找一种替代家兔热原试验的方法 ,最近我们对用THP 1细胞系检测热原的可行性进行了初步探索。其原理是 :将细菌内毒素标准品刺激THP 1细胞 6h后 ,用ELISA双抗夹心法测定其释放的内源性热原 (TNF α)。试验结果表明 :TNF α的量与内毒素的量在一定范围 (0 .32EU mL— 2 0 0EU mL)内有很好的相关性 ,两种不同来源的内毒素得到的结果相似。说明该方法作为替代家兔热原检查的新方法有一定的可行性

关键词: 热原, 家兔热原试验, 细胞因子, THP1, TNFα