Abstract: Objective The SPF rabbits breeding stock is established for our country, it can meet the needs of scientific research and production and test. Method The rabbits are cleaned by cesarotomy, the young rabbits are reared in isolator by artificial brease feed. The young rabbits to weaned are given 5 varieties normal intestinal bacteria and then transfer them into the barrier system. Result At present, there are about 100 SPF rabbits in the barrier system. We have examined microorganism of the rabbits, they are accorded with the standard of SPF rabbits of China.

Key words: Cesarean section, Breast feeding, Rabbits

摘要： 目的　对普通级实验家兔进行了剖腹净化 ,建立我国SPF家兔种子核心群 ,使普通级兔子升级换代 ,以满足科研、生产及检定工作不断发展的需要。方法　采用剖腹摘取子宫术 ,在无菌隔离器内以吮吸法人工哺乳仔兔 ,离乳后将正常菌群植入仔兔体内 ,并转入SPF屏障设施内饲养、繁殖。结果　建立了具有 10 0只种兔的SPF兔子核心群 ,兔群生长发育正常。经多次微生物检测 ,符合SPF标准。SPF兔群将作为建立SPF实验用兔生产群的核心种群。

关键词: 剖腹产术, 母乳喂养, 兔子