Abstract: The study was to finding out the effect of Superovulation on hormone to Wistar rat. and the result of embryo transfer. Injecting different dosage PMSG and hCG to female rat, then each were collected from the oviducts of the plugged females. Then transferred to oviducts of pseudo pregnant recipients. The eight cell embryos were collected from the womb of the plugged females then transferred to womb of precidopregnant recipients. The results indicated that 50 IU PMSG and hCG can make rat's superovulation best; can average collect 36.3±15 8 two cell embryos, the rate of full_time young was 73 33%;can average collect 22 6±13 1 eight cell embryos, the rate of full_time young was 75 00%.

Key words: Superovulation, Transfer, Embryos, Rat

摘要： 不同剂量激素对Wistar雌鼠超数排卵 ,并与正常雄鼠交配 ,收集 2 -细胞胚胎进行输卵管移植 ,收集 8-细胞胚胎进行子宫移植 ,结果发现每只Wistar雌鼠间隔 4 8h分别腹腔注射 5 0IU的PMSG与hCG ,Wistar大鼠的超数排卵效果最好 ,平均可获得 2 -细胞胚胎 36 .3± 15 8枚 ,输卵管移植后产仔率为 73 33% ,平均可获得 8-细胞 2 2 6±13 1枚 ,子宫移植产仔率为 75 0 0 %。

关键词: 超数排卵, 移植, 胚胎, 大鼠