Abstract: Objective To explore the induction of estrus synchronization in SD rats and BALB / c mice females by progesterone injection. Method Progesterone hormone was injected into 8 weeks SD rats and BALB / c mice females on the first and second day of the experiment at doses of 10 mg / rat and 2 mg / mouse respectively, and cohabited on the fourth day. The observations of copulatory plugs were performed on the fifth, sixth and seventh day of the experiment. Embryo transfer was performed in copulatory plug checked pseudopregnant SD rats, and pseudopregnant rats obtained by mating with vasoligated males were used as controls. Blastocysts were flushed from embolized BALB / c mice females for embryonic stem (ES ) cell injection. Result The highest copulatory plug rate of SD rats females injected with progesterone reached 44% on a single day and more than 80% overall. The highest copulatory plug rate of BALB / c mice females injected with progesterone reached 46% on a single day and more than 70% overall. The copulatory plug rate of progesterone injected group was 7 - 8 times higher than traditional method group on a single day, and more than 15 times higher than traditional method group overall. Compared with the traditional method, in progesterone injected group, the colony population of SD rats females and BALB / c mice females could be reduced to 10% - 20%; the embryo birth rate of SD rats pseudopregnant could increase by about 13%. Conclusion Progesterone injection 10 mg / rat and 2 mg / mouse can be used for estrus synchronization in 8 weeks SD rats and BALB / c mice females respectively, and can significantly increase the copulatory plug rate and SD rats embryo birth rate.

Key words: progesterone, embryo transfer, estrus synchronization, SD, BALB / c, pseudopregnant rat, blastocyst donor

摘要： 目的 探索采用注射孕酮的方式诱导 SD 大鼠和 BALB / c 小鼠的雌鼠同步发情。 方法 在实验第 1 天和第 2天给 8 周龄 SD 大鼠和 BALB / c 小鼠的雌鼠注射孕酮激素和注射孕酮量分别为每只 10 mg 和 2 mg,连续注射 2 d,第4 天合笼,分别在第 5 天、第 6 天和第 7 天进行检栓。 利用见栓后的 SD 大鼠假孕鼠进行胚胎移植,选择发情雌鼠与结扎雄鼠交配得到的假孕鼠同步移植作为对照;利用见栓后的 BALB / c 小鼠雌鼠冲取囊胚用于胚胎干细胞( ESC)注射。 结果 注射孕酮后的 SD 大鼠雌鼠单日最高见栓率可达到 44%,整体见栓率能达到 80%以上;注射孕酮后的BALB / c 小鼠雌鼠单日最高见栓率可达到 46%,整体见栓率能达到 70%以上;注射孕酮单日见栓率是传统选择发情后合笼见栓的 7 ~ 8 倍,整体见栓率是传统方式的 15 倍以上,与传统方法相比,用于准备假孕的 SD 大鼠雌鼠和供体囊胚 BALB / c 小鼠雌鼠的基础雌鼠数量可降低 10% ~ 20%;SD 大鼠假孕鼠的胚胎出生率较传统方法提高 13%。结论 注射每只 10 mg 和每只 2 mg 孕酮可分别用于诱导 8 周龄 SD 大鼠雌鼠和 BALB / c 小鼠的雌鼠同步发情,可显著提高见栓率和 SD 大鼠雌鼠胚胎出生率。

关键词: 孕酮, 胚胎移植, 同步发情, SD, BALB / c, 假孕鼠, 囊胚供体