Abstract: Objective Establishing CD3 monoclonal antibody to induce humanized cytokine storm mice model. Method The peripheral blood mononuclear cells (PBMCs) of healthy adults were extracted, and NSG mice aged 10-12 weeks after intravenous injection were randomly divided into 2 groups: (1) PBMCs group (2) OKT3 (CD3 monoclonal antibody, anti-CD3 mAb, 0. 1 mg / mL) + PBMCs. The experimental group was injected with CD3 antibody 0. 5 mg / kg 6 days later, and serum TGF-β, CTLA-4, IL-10, IL-2, INF-γ, IL-7, and IL-6 levels were detected by ELISA 6 hours later. Result Compared with the control group, the use of OKT3 can effectively induce the cytokine storm IL-2 (P<0. 001), INF-γ (P<0. 001), TGF-β (P<0. 001), IL-6 ( P < 0. 001), IL-7 ( P < 0. 001) and CTLA-4 ( P < 0. 001). Conclusion The cytokine storm NSG mouse model constructed this time is conducive to the further study of the disease.

Key words: cytokine storm, INF-γ, CTLA-4, IL-7

摘要： 目的 构建 CD3 单克隆抗体诱导人源化细胞因子风暴小鼠模型。 方法 提取健康成人的外周血单核细胞( PBMCs) ,静脉注射 10 ~ 12 周龄 NSG 小鼠后,随机分为 2 组:( 1) PBMCs 组 ( 2) OKT3 ( CD3 单抗,anti-CD3 mAb, 0. 1 mg / mL) +PBMCs。 实验组 6 d 后注射 CD3 抗体 0. 5 mg / kg,6 h 后 ELISA 检测血清 IL-10、CTLA-4、INF-γ、IL-6、IL-2、IL-7 和 TGF-β 水平。 结果 相比对照组,使用 OKT3 能有效诱导出细胞因子风暴,其中 IL-2(P<0. 001) 、INF-γ(P<0. 001) 、TGF-β(P<0. 001) 、IL-6(P<0. 001) 、IL-7( P<0. 001) 和 CTLA-4( P<0. 001) 。 结论 成功构建细胞因子风暴 NSG 小鼠模型。

关键词: 细胞因子风暴, INF-γ, CTLA-4, IL-7