关键词: 角膜移植排斥, 动物模型, 并发症, 排斥率

Abstract: To analyze the causes for complications during surgical construction of rat penetrating keratoplasty model, to discuss how to prevent and manage these complications and to summarize experience and skills to increase rejection rate for this allografts model. In order to construct an orthotopic rat penetrating keratoplasty model, 34 allografts (SD→Wistar) were performed in the study group and 34 autografts (Wistar→Wistar)were used as controls. To increase rejection rate of allografts, the donor corneas were trephined eccentrically and the sutures were not removed on the recipients. No rejection was found on the autografts, while 100% rejection was identified in the allografts, with an average survival time at 10.8±2 1 days. Complications were encoutered in 10 eyes, which included iris prolapses, graft cleavage, hemorrhage in the anterior chamber, cataract and infection. Conclusion:MHC mismatched species, eccentrically removed donor corneas and kept sutures in the recipients contribute to the high rate of rejection. Gentle and skilled surgical manipulations, sharp instruments and pupil enlargement are of great importance to successful operations.

Key words: Corneal transplant rejection, Animal model, Complication, Rejection rate